长沙地区两种常见蚯蚓及太平Ⅱ号蚓的EST、LDH同工酶研究  

On the Electrophoresis of EST and LDH isozyme of ordinary Earthworm in Changsha and E.foetida

作　　者：郭永灿[1] 王振中[1] 张友梅[1] 赖勤[1] 毛东文 王淑云 

机构地区：[1]湖南师范大学生物学系

出　　处：《激光生物学报》1997年第4期1235-1240,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金

摘　　要：本文报道了长沙地区两种常见蚯蚓——日本杜拉蚓、舒脉环毛蚓以及人工养殖的太平Ⅱ号蚓为实验材料，应用聚丙烯酰胺凝胶圆盘电泳方法，分析ＥＳＴ、ＬＤＨ两种同工酶。实验结果表明ＥＳＴ同工酶酶谱复杂，ＬＤＨ同工酶具多态现象。ＥＳＴ同工酶能正确反映出种属间的差异。Using the polyacry lamide gel disc electrophoresis technique,we have analyzed two kinds of isozyme(EST isozyme and LDH isozyme)which are extracted from two kinds of ordinary earthworm in changshaDrawida jopomca,Pheretima schmardae,and raised Eisenia foetida.The results have been shown as follows:1.EST isozyme is relatively complicated,2.LDH isozyme hes polymorphism.Electrophorogram of EST isozyme sccumately reflects speciesgeners.specificnies therefore it can be used as a good biochemical index on sigochaeta taxomomy.

关 键 词：蚯蚓 酯酶 乳酸脱氢酶 同功酶 

分 类 号：Q959.193[生物学—动物学]