系统性红斑狼疮外周血单个核细胞蛋白组学的初步研究  

Proteomic analysis of peripheral blood mononuclear cells from patients with systemic lupus erythematosus

作　　者：周迪[1] 郝飞[1] 夏汝山[1] 牟芝蓉[2] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆400038 [2]第三军医大学全军免疫研究所,重庆400038

出　　处：《实用医院临床杂志》2008年第3期35-38,共4页Practical Journal of Clinical Medicine

基　　金：重庆市西南医院创新基金资助项目(编号:200716)

摘　　要：目的探讨系统性红斑狼疮(SLE)外周血单个核细胞(PBMC)蛋白质组的变化。方法分别提取14例SLE患者及9例健康献血者的PBMC,采用双向电泳技术分离全蛋白质组,对部分差异的蛋白质点进行离子阱高效液相色谱/质谱分析,测定其肽质量指纹图谱,与互联网蛋白质数据库的数据进行比对。结果获得了较好的双向电泳图像,图像分析显示:1例SLE患者、4例健康献血者PBMC蛋白匹配点为1084个,其中SLE患者高表达及特异表达蛋白点为71个及17个,健康献血者高表达及特异表达蛋白点为45个及20个,与所有实验2-DE图谱逐个比对后,对表达差异重复性高的30个蛋白质点进行肽质量指纹图谱分析,经数据库检索后,鉴定出2个胞浆蛋白质酪氨酸转运核糖核酸合成酶(tyrosyl-tRNA synthetase)和斑联蛋白(zyxin)在SLE患者的PBMC中低表达。结论SLE患者PBMC蛋白组学研究可能为深入研究其发病机制、建立新的诊断指标提供一个良好的平台。Objective To investigate variations in protein expression of peripheral blood mononuelear cells （PBMC） in the course of systemic lupus erythematosus. Methods PBMC protein samples were extracted from 14 SLE patients and 9 healthy controls. The proteins were separated by immobilized pH gradient two-dimensional （2-D） gel eleetrophoresis. The 2-D electrophoretogram was analyzed using PDquest7. 1 software. The differential spots were identified by lon Trap high performance liquid chromatography-Chip Mass spectrum （HPLC-Chip-MS/MS） and database searching was performed on the internet. Results The 2-D eleetrophoretogram was stable and showed 1084 match spots in 1 SLE sample and 4-in-1 healthy control sample, of which 71 and 17 proteins were overexpressed or unique in SLE group. While in the control, the data were replaced by 45 and 20 respectively. We compared these match spots one by one and made the reproducible 30 ones selected and through the progress of HPLC-Chip-MS/MS. Two of them were identified primarily. They were tyrosyl-tRNA synthetase and zyxin, of which the expression was suppressed in patients suffered from SLE. Conclusion The study methods of proteome on PBMC of SLE may make some contributions to pathogenesis study and foundation of new diagnosis criterion

关 键 词：系统性红斑狼疮 外周血单个核细胞 蛋白质组 酪氨酸转运核糖核酸合成酶 斑联蛋白 

分 类 号：R593.241[医药卫生—内科学]