Bti的溶血特性及其在Bti制剂毒力检测中的应用  

RESEARCH ON THE HAEMOLYTIC METHOD OF BACILLUS THURINIENSIS SUBSP ISRAELENSIS(BTI) AND ITS APPLIANCE FOR TOXICITY DETECTION OF BTI PREPARATIONS

作　　者：但汉斌[1] 陈勇强[1] 

机构地区：[1]天津农科院园艺所农业微生物研究室,天津300192

出　　处：《微生物学通报》1997年第6期326-330,共5页Microbiology China

摘　　要：实验利用Bti(苏云金杆菌以色列亚种）对兔红细胞（RBC）的溶血特性，以A541特征光吸收对Bti毒蛋白溶液体积或其对数作回归分析。结果表明：二者具有良好的直线关系。相关系数r≥0．95。方差分析表明：不同梯度的Bti每蛋白溶液处理之间差异极显著，处理内各重复之间差异不显著，同一天内的重复检测没有显著差异。本又给出了Bti制剂毒力检测的参考程度，并就该程度应用于Bti毒力快速检测作了较为详尽的探讨。Impure crystal protein of Bti ferment is disolved to thaw out the rabit red boloodcell(RBC) and solution of which is detected on the spectrophotometer for A541 data. Followingregression analysis, A54l is found to be in good linear correlation with the volume(or its logscale) of Bti ICP solution, coefficient of correlation(r)≥0. 95. Variance analysis results thatdifferent concentration of Bti ICP solution gives cleariy diffent A541 data, but the sameconcentration gives the similar A541 data(F test shows no clearly variance) even with 6 timesrepeats. Repeat exam of the same day gives no clearly variance from F tests. Referenceprocedure of Bti preparation toxicity detection is also given out in this paper.

关 键 词：苏云金杆菌 以色列亚种 溶血实验 毒力检测 

分 类 号：S482.7[农业科学—农药学]