中国脊髓灰质炎Ⅱ型疫苗相关分离株病毒性状的观察  

AN OBSERVATION ON MOLECULAR CHARACTERISTICS OF CHINA VACCINE-RELATED TYPE Ⅱ POLIOVIRUS

作　　者：侯晓辉[1] 张礼璧[1] T.Yoneyama K.Yoshii H.Shimizu H.Yoshida 李杰[1] 郑红[1] 方勇[1] 朱宏[1] M.Hara Akio Hagiwara 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1997年第4期332-338,共7页Chinese Journal of Virology

基　　金：国家自然科学基金;卫生部科研基金

摘　　要：对１９９４年中国分离的１３株脊髓灰质炎Ⅱ型疫苗相关株进行了ＰＣＲ－ＲＦＬＰ分析，发现７株为重组病毒，毒力较疫苗株有回复。在Ⅱ型脊髓灰质炎病毒基因序列上，对于神经毒力有重要影响的第４８１位核苷酸发生了突变（Ｃ→Ｇ），另一个被视为重要位点的第２９０８位核苷酸无一发生变化，反而在第２９０９位核苷发生了高频率的点突变，意味着２９０９位点在中国Ⅱ型疫苗相关株的自然变异中可能起着重要作用。根据转基因小鼠实验和温度敏感实验（Ｒｃｔ－ｍａｒｋｅｒ），脊髓灰质炎病毒的神经毒力大小并不与温度敏感实验所显示的毒力特征呈正相关性。Seven of the thirteen vaccine-related type Ⅱ polioviruses isolated in China were identified as recombinant strains by PCR-RFLP and sequencing analysis was made in 1994. With Transgenic mice test and Temperature-sensitive multiplication test (Rct-marker), most of them showed higher neurovirulence and higher ability of growth at 39.8℃ than Sabin2 vaccine strain but lower than that of MEF strain. However, the neurovirulence of the 7 isolates is not completely correlated with biological characteristics of Rct-marker. Partial genomic sequences of 5′non-coding region and VP1 region of these viruses were also examined. It was found that all recombinants have a transition from C to G at neucleotide position 481, some of them have T to A or T to C transition at position 2909, but non of them has mutation at position 2908 which is considered previously as one of the important points with type Ⅱ poliovirus neurovirulence.

关 键 词：脊髓灰质炎 病毒 疫苗 神经毒力 

分 类 号：R512.403[医药卫生—内科学]