新疆乌鲁木齐HIV-1流行毒株膜蛋白基因C2-V3区序列测定和亚型分析  

SUBTYPE AND SEQUENCE ANALYSIS OF THE C2-V3 REGION OF GENES OF MEMBRANE PROTEIN AMONG HIV-1 STRAINS IN WULUMUQI EPIDEMIC AREA OF XINJIANG

作　　者：白旭华[1,2,3,4] 管永军[1,2,3,4] 张远志[1,2,3,4] 邵一鸣 王冬莉[1,2,3,4] 苏玲[1,2,3,4] 刘小良[1,2,3,4] 赵全壁[1,2,3,4] 杜大卫 JosefKostler HansWolf 

机构地区：[1]新疆维吾尔自治区卫生防疫站 [2]中国预防医学科学院艾滋病参比实验室 [3]新疆乌鲁木齐市卫生防疫站 [4]德国累根斯堡大学医学微生物学研究所

出　　处：《病毒学报》1997年第4期339-344,共6页Chinese Journal of Virology

摘　　要：应用ＰＣＲ方法对７份１９９６年３－５月采集于新疆乌鲁木齐ＨＩＶ－１阳性静脉吸毒者的外周血单核细胞（ＰＢＭＣｓ）样品进行扩增，获得了ＨＩＶ－１膜蛋白（ｅｎｖ）基因的核酸片段，并对其Ｃ２－Ｖ３区及邻区３５０－４５０个核苷酸序列进行了测定和分析。研究结果表明，７份样品都为Ｃ亚型的ＨＩＶ－１毒株序列，其组内的基因离散率为１．０％；与Ａ－Ｅ参考亚型及部分Ｃ亚型代表株序列相比较，这７个毒株与代表印度Ｃ亚型毒株及云南德宏州Ｃ亚型毒株的序列十分相似，其组间的基因离散率均为３．５％，与非洲Ｃ亚型毒株ｎｏｆ的基因离散率为１４．０％。根据以上数据及其它资料提示，ＨＩＶ－１在新疆乌鲁木齐的流行时间不长，且其毒株的传入与流行在云南德宏州的相同亚型ＨＩＶ－１毒株密切相关。Seven DNA fragments of HIV-1 env genes were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from HIV-1 sero-positive drug injecting users (IDUs) in Xinjiang. The C2-V3 region (about 450bp) of them were sequenced. Sequence analysis showed that they are HIV-1 subtypes C, with 1.0% gene divergence among them. The 7 subtype C strains are most close to those found in India and Ruili county of Yunnan, and their nucleotide sequence divergence are both 3.5%. The low level of sequence divergence suggests a recent epidemic of HIV-1 subtype C strain.

关 键 词：亚型分析 序列测定 艾滋病毒-1 毒株 膜蛋白基因 

分 类 号：R373.9[医药卫生—病原生物学]