使用生物纳米磁珠对低浓度目标细菌的磁捕获  

Using biofunctional magnetic nanoparticles to capture targeted bacteria at low concentration

作　　者：田菊霞[1] 解宇[1] 楼忠明[2] 杨艳宏[1] 王文香[2] 

机构地区：[1]杭州师范大学医学院,310018 [2]杭州市萧山区第一人民医院

出　　处：《浙江医学》2008年第4期332-334,共3页Zhejiang Medical Journal

基　　金：浙江省自然科学基金资助项目(Y205373);杭州市卫生科技重点资助项目(2005Z004);杭州市科技引导项目(20051333B37)

摘　　要：目的以从磁性细菌体内提取的纳米磁珠为载体,对低浓度大肠杆菌O157:H7进行快速磁捕获与分离。方法从磁性细菌中获取生物纳米磁珠,然后将大肠杆菌O157抗体接种于该磁珠表面,形成免疫磁珠后,对细菌混合液中浓度约为1cfu/10ml的大肠杆菌O157:H7进行快速磁捕获与分离。结果菌源性纳米磁珠表面大肠杆菌O157抗体的包被固定量为132μg/mg磁珠。当大肠杆菌O157免疫磁珠浓度为50μg/ml以上时,即有较好的捕获和分离效果,而且特异性较高,显著强于传统的离心分离法(P<0.01),当浓度达到100μg/ml以上时捕获和分离效果更好。结论利用磁性细菌由来的生物纳米磁珠,可将目标细菌抗体包被于生物纳米磁珠表面,制成免疫磁珠后,通过外磁场诱导可快速、准确地进行低浓度目标细菌的捕获与分离。Objective To extract magnetic nanoparticles from magnetic bacteria and to capture and isolate Escherichia coil O157：H7 （E-coil O157：H7） at low concentration. Methods Magnetic nanoparticles were extracted from magnetic bacteria. The extracted bacterial magnetic particles （BMPs） were coupled to anti-Escherichia coil O157：H7 to form immunomagnetic particles, by which E-coil O157：H7 （1cfu/10ml） in bacterial mixture was isolated. Results The quantity of anti-E-coli O157：H7 coated to BMPs was 132 μg/mg. The targeted E-coil O157：H7 was quickly separated by using immunomagnetic particles of 100μg/ml in 10ml bacterial mixture. Conclusion The targeted bacteria at low concentration can be rapidly captured and isolated by immunomagnetic particles prepared in the study.

关 键 词：磁性细菌 纳米磁珠 捕获 分离 

分 类 号：R446.5[医药卫生—诊断学]