阿霉素脂质体的制备及其体外细胞毒活性  

Preparation of adriamycin-liposome and its cytotoxicity on human ovarian carcinoma SKOV3 cells in vitro

作　　者：赵梦丹[1] 俞飞江[2] 虞和永[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,杭州310006 [2]浙江省肿瘤医院

出　　处：《浙江医学》2008年第4期337-339,F0003,共4页Zhejiang Medical Journal

摘　　要：目的建立一种阿霉素脂质体的制备方法,并观察所制备的阿霉素脂质体体外对人卵巢癌细胞SKOV3的杀伤能力。方法在pH梯度法的基础上,运用多次超声乳化法制备阿霉素脂质体,然后采用交联葡聚糖G50凝胶柱进行分离。以荧光分光光度法测定阿霉素脂质体包封率,四唑盐比色法测定细胞抑制率,荧光倒置显微镜观察人卵巢癌细胞SKOV3生长抑制情况。结果阿霉素脂质体粒径为70～300(平均139)nm,包封率为90.9%。在10、2、0.4、0.08、0.016μg/ml浓度点,阿霉素脂质体对人卵巢癌细胞SKOV3的抑制率分别为79%、64%、46%、30%、15%,游离阿霉素抑制率分别为70%、56%、37%、25%、11%,相比之下阿霉素脂质体各浓度点的抑制率更高。此外,转染后细胞荧光染色显示,阿霉素脂质体大部分集中在细胞内,而游离阿霉素只能部分进入细胞膜。结论此法制备的脂质体包封率高,简便易行,重现性好,而且对人卵巢癌细胞SKOV3具有高度的杀伤活性。Objective To establish the method for preparation of adriamycin-liposome and to observe its cytotoxicity on human ovarian carcinoma SKOV3 cells in vitro. Methods Adriamycin-loposome was prepared by ultrosonic agitation method and purified by exclusion chromatography with Sephadex G-50. The optimal wavelength and encapsulation rate of adriamycin-liposome was investigated by fluorometer. The cytotoxicity of adriamycin-liposome was evaluated by MTT assay and invert fluorescence microscope. Results The encapsulation rate of adriamycin was 90.9%. The cytotoxicity of adiamycin-liposomes on SKOV3 was similar to that of free adriamycin, however, the effect lasted longer. Conclusion The preparation method of adriamycin-liposome in this study is practicable one with high encapsulation efficiency and cytotoxic activity.

关 键 词：阿霉素脂质体 包封率 细胞毒性 

分 类 号：R943[医药卫生—药剂学] R96[医药卫生—药学]