氟对体外培养乳鼠软骨细胞增殖的影响  

Influence of fluoride on proliferation of newborn mouse chondrocytes

作　　者：李良忠[1] 钟近洁[2] 许永华[3] 刘开泰[4] 刘继文[5] 张东辉[3] 李建瑛[3] 是文辉[3] 

机构地区：[1]北京大学医院口腔中心,100871 [2]新疆医科大学组胚教研室 [3]兰州军区乌鲁木齐总医院动物实验科 [4]中国疾病预防控制中心教育培训处 [5]新疆医科大学公共卫生学院

出　　处：《中国地方病学杂志》2008年第3期264-267,共4页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金（30560131）

摘　　要：目的探讨氟对体外培养乳鼠软骨细胞增殖的影响。方法采用细胞培养的方法，原代培养昆明乳鼠软骨细胞，传第3代后按染氟剂量不同分为0（对照）、5、10、20、40mg／L组，10d后光、电镜观察软骨细胞形态学变化；采用生长曲线、噻唑蓝（MTT）方法，在染氟24、48、72h测定细胞数量变化及细胞增殖率。结果染氟10d后，镜下0、5、10mg／L组软骨细胞表现为增殖，细胞生长旺盛，均可见部分细胞核中核仁数增加；40mg／L组部分软骨细胞中有染色质固缩或凝结成块状，可见到凋亡细胞。在染氟24h，细胞增殖活力各染氟组组间比较差异无统计学意义（F=2．313，P〉0．05）。在染氟48、72h，0mg／L组[（23．5±4．6）％、（29．9±1．7）％]、5,w／L组[（34．6±4．7）％、（45．3±5．9）％]、10mg／L组[（39．9±4．8）％、（56．8±5．5）％]、20mg／L组[（31．8±4．1）％、（38．3±6．5）％]、40mg／L组[（28．3±4．3）％、（33．4±4．8）％]组间比较差异有统计学意义（F值分别为11．401、25．671，P〈0．05）；各染氟组细胞增殖活力与对照组比较差异均有统计学意义（P〈0．05），其中5、10mg／L组明显高于40mg／L组（P〈0．05）。结论低剂量氟在较短作用时间内可以促进体外培养小鼠软骨细胞的增殖，剂量升高时表现为抑制。Objective To study the proliferation of in vitro cultured mouse chondrocytes exposed to different doses of fluoride. Methods The third generation of primary cultured chondrocytes were exposed to the concentrations of 0, 5, 10, 20, 40 mg/L fluoride for 10 days to observe the morphological changes under light microscope and electron microscope to counter the numbers of chondrocytes and proliferating rate with the growth curve and MTT. Results After exposed to fluoride for 10 days, the proliferation was present in the chondrocytes of the 5,10,20 mg/L groups, and shrinked chromatine and apoptosed chondrocytes were seen in 40 mg/L group .The absorbance was not significantly different between all groups（F = 2.313 ,P 〉 0.05） ; after exposed to fluoride for 48 and 72 hours,there was a significant difference of proliferating ability among 0 mg/L（the control）group[ （23.5 ± 4.6）%, （29.9 ± 1.7）%], 5 mg/L group[ （34.6 ± 4.7）%,（45.3 ± 5.9）%], 10 mg/L group[ （39.9 ± 4.8）%,（56.8 ± 5.5）%], 20 mg/L group[ （31.8 ±4.1）%,（38.3 ± 6.5）%] and 40 mg/L group[（28.3 ± 4.3）%,（33.4 ± 4.8）%] （F = 11.401, 25.671 ,P 〈 0.05）. There was a significant difference compared with the control group （P 〈 0.05） with that of 5 and 10 mg/L groups higher than that of 40 mg/L groups （P 〈 0.05）. Conclusions Lower doses of fluoride improve the proliferation of in vitro mouse chondrocyte in a short exposing time, higher doses result in the opposite.

关 键 词：氟 软骨细胞 细胞增殖 

分 类 号：R686[医药卫生—骨科学]