氟致牛外周血淋巴细胞凋亡及DNA损伤的效应观察  

Effect of fluoride on apoptosis and DNA damage in ox peripheral blood lymphocytes

作　　者：王敏[1,2] 邢厚娟 朱绯[1] 徐世文[1] 

机构地区：[1]东北农业大学内科教研室,哈尔滨150030 [2]黑龙江省畜牧兽医职业学院传染病教研室

出　　处：《中国地方病学杂志》2008年第3期272-275,共4页Chinese Jouranl of Endemiology

摘　　要：目的探讨氟化钠（NaF）致体外培养的黄牛外周血淋巴细胞损伤和诱导细胞凋亡的作用。方法应用淋巴细胞分离液分离黄牛外周血淋巴细胞，将不同剂量NaF[0（对照组）、4、8、12、16mg／L]加入培养体系中，培养24h后，采用四甲基偶氮唑盐（MTT）比色法检测细胞活性，吖啶橙／溴化乙锭（AO／EB）染色法观察细胞核变化，琼脂糖凝胶电泳法检测DNA断裂情况，碱性单细胞凝胶电泳（SCGE）检测DNA损伤情况。结果4、8、12、16mg／L组淋巴细胞的存活率分别为（73．743±0．552）％、（69．184±0．724）％、（65．736±01055）％、（63．651±0．287）％，较对照组[（100．000±0．003）％]明显下降（P〈0．01）。观察到特征性凋亡细胞及有明显的DNA损伤，呈明显的剂量-效应关系（R^2=0．7456）。结论一定剂量的NaF有致牛外周血淋巴细胞损伤和凋亡的作用。Objective To explore the effect of sodium fluoride on DNA damage and apoptosis on ox peripheral blood lymphocyte cultured/n vitro. Methods Using lymphoeytes separation medium lymphoeytes were separated and different concentration of NaF（0,4,8,12,16 mg/L） were added into the cultual system of lymphoeytes for 24 h. Cell viability was measured by MTT, nuclear changes stained by acridine orange-ethidium bromine staining （AO/EB） were observed under fluoroscope, DNA fragment was measured by agarose gel dectrophoresis. DNA damage was detected by alkaline SCGE. The differences between each groups were compared. Results Cells were exposed to 4, 8, 16 and 24 mg/L NaF in 24 h, cell survival rates, respectively being （73.743 ± 0.552）%, （69.184 ±0.724）%, （65.736 ± 0.055）% and （63.651 ± 0.287）%, decreased significantly compared to control group. There were distinctive cell apoptosis, evident DNA damage and visible dose-effect relation（R^2 = 0.7456）. Conclusions A certain concentration of sodium fluoride leads to lymphoeytes apoptosis and DNA damage.

关 键 词：氟化物 淋巴细胞 DNA损伤 细胞凋亡 

分 类 号：R686[医药卫生—骨科学]