抗幽门螺杆菌单克隆抗体与人血小板交叉识别的实验研究  

Experimental study on the cross-antigens between the human platelet and Helicobacter pylori

作　　者：白艳艳[1] 王兆钺[1] 缪竞诚[2] 白霞[1] 阮长耿[1] 

机构地区：[1]苏州大学附属第一医院江苏省血液研究所卫生部血栓与止血重点实验室,苏州215006 [2]苏州大学基础医学系,苏州215007

出　　处：《中国免疫学杂志》2008年第5期448-451,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(30770917);江苏省卫生厅“135工程”血液重点学科开放课题(K0607);苏州大学博士学位科研启动基金

摘　　要：目的:研究抗幽门螺杆菌(Hp)尿素酶B(ureB)单克隆抗体与血小板膜糖蛋白(GP)的结合情况。方法:运用Western blot、流式细胞术(FCM)和免疫放射法(IRMA)分析血小板膜GP成分与幽门螺杆菌ureB成分的相关性。结果:抗幽门螺杆菌ureB单抗1F11与血小板成分GPⅢa、P-选择素有一定程度结合,但与GPⅠb、GPⅡb及GPⅥ无结合。结论:血小板成分GPⅢa、P-选择素与幽门螺杆菌ureB存在交叉识别的共同抗原表位,提示Hp感染与部分特发性血小板减少性紫癜(ITP)的发病机理有关。Objective： To study on binding capacity of the monoclonal-antibody against Helicobacter pylori ureB combined with glycoproteins of platelet membrane.Methods： The relativity between GPs and ureB was identified with the methods of Western blot, FCM and IRMA.Results： McAb to Helicobacter pylori ureB -1F11 could bind with GPⅢa or other kinds of protein （such as P-selectin） on platelet membrance, but not with GPⅠb, GPⅡb and GPⅥ. Conclusion： Our results suggest that there might be cross-reacting antigens between GPⅢa or P-selectin of platelets and Helicobacter pylori ureB, and that the pathogenesis of some ITP should be related with the infection of Helicobacter pylori.

关 键 词：血小板 糖蛋白 幽门螺杆菌 尿素酶B 交叉抗原 

分 类 号：R392.1[医药卫生—免疫学]