瘦素体外对人卵巢黄素化颗粒细胞雌、孕激素分泌的影响  

The effects of leptin on estradiol and progesterone production by human luteinized granulose cells in vitro

作　　者：吕立群[1] 刘义[1] 孙永玉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科

出　　处：《中国妇幼保健》2008年第15期2119-2121,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨瘦素体外对人卵巢黄素化颗粒细胞雌激素(E2)和孕激素(P)分泌的影响。方法:将来自体外受精-胚胎移植(IVF-ET)的卵巢黄素化颗粒细胞纯化后,在不同的浓度瘦素(0、10、20、100 ng/ml)和FSH(0、1、5、10 IU/ml)单独或联合作用下进行体外培养48 h,收集培养液,用放射免疫方法测定颗粒细胞产生E2及P的浓度。结果:①不同浓度的瘦素对黄素化颗粒细胞E2和P的基础分泌功能无明显影响;②FSH能促进黄素化颗粒细胞E2和P的分泌,随FSH浓度增加,E2和P的水平逐渐增高;③不同浓度瘦素对FSH促进黄素化颗粒细胞E2的分泌均有抑制作用,随瘦素浓度增加,抑制作用逐渐增强,但对P的分泌无影响。结论:瘦素抑制FSH刺激卵巢黄素化颗粒细胞雌激素的分泌,高浓度瘦素可能影响卵泡发育和黄体形成。Objeclive： To investigate the effects of leptin on estrogen （E2） and progesterone （P） secretion of human luteinized granulose cell in vitro. Methods： Human luteinized granulose cells from In Vitro Fertilization -Embryo Transfer （IVF- ET） were purified and then cultured with medium plus various concentration of leptin （0, 10, 20, 100 ng/ml） and FSH （0, 1, 5, 10 U/ml） or alone for 48 h. The media were collected to measure the concentration of E2 and P by radioimmunoassay. Results： ①Addition of different concentra- tions of leptin had no significant effect on basal E2 and P production by luteinized granulose cells. ② FSH could cause a concentration - dependent increase of E2 and P production by luteinized granulose cells, the level of E2 and P gradually increased with the increasing of FSH concentration. ③ Different concentrations of leptin caused a dose - dependent inhibition of FSH - stimulated E2 production by luteinized granulose cells, but leptin had no effect on FSH - stimulated P production. Conclusion： Effects of leptin can inhibit FSH - stimulated E2 and P production by luteinized granulose cells. High levels of leptin can affect the development of follicle and luteal.

关 键 词：瘦素 颗粒细胞 雌激素 孕激素 

分 类 号：R711.6[医药卫生—妇产科学]