红褐斑腿蝗卵黄蛋白的分离纯化及性质分析  

Purification and characterization of vitellin from locust Catantops pinguis (Stl) (Orthoptera:Catantopidae)

作　　者：金建雅[1] 王世贵[1] 徐彩娣[1] 黄淑娟[1] 

机构地区：[1]杭州师范大学动物科学与技术杭州市重点实验室,杭州310036

出　　处：《昆虫学报》2008年第4期372-378,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金资助项目(30300042)

摘　　要：用蒸馏水沉淀法、凝胶过滤、蛋白质电泳等方法提取纯化了红褐斑腿蝗Catantops pinguis(Stl)的卵黄蛋白,并对其性质进行了分析。电泳结合不同的染色方法证明红褐斑腿蝗的卵黄蛋白为一种糖脂复合蛋白,其分子量约为548kD,由7个亚基组成,亚基分子量分别为147.3,100.2,95.9,59.6,53.6,49.0和42.2kD。卵黄蛋白经高效液相色谱分析检测到17种氨基酸和NH3峰,其中谷氨酸(Glu)百分含量最高,达13.46%,天冬氨酸(Asp)、亮氨酸(Leu)、半胱氨酸(Cys)、精氨酸(Arg)含量比较高,脯氨酸(Pro)、组氨酸(His)、赖氨酸(Lys)含量较低。The vitellin was purified from eggs of the precipitation, gel filtration and gel electrophoresis. Its locust Catantops pinguis （Stal） using distilled water properties were also analyzed using polyacrylamide gel electrophoresis and different dyeing methods. The results indicated that the vitellin of C. pinguis was a kind of protein containing sugar and fat. The molecular weight of the vitellin was 548 kD, consisting of seven subunits with molecular weight of 147.3, 100.2, 95.9, 59.6, 53.6, 49.0 and 42.2 kD, respectively. From the vitellin, 17 kinds of amino acids and NH3 peaks were detected by HPLC, among which the content of Glu was highest with 13.46% in the vitellin, and the contents of Asp, Leu, Cys and Arg were relatively high, while contents of Pro, His and Lys were low.

关 键 词：红褐斑腿蝗 卵黄蛋白 分离纯化 性质 

分 类 号：Q966[生物学—昆虫学]