根结线虫生防木霉菌菌株Snef85生物学特性初步研究  

Biological Characters of Trichoderma harzianum for Bio-control Meloidogyne spp.

作　　者：靳莹莹[1] 段玉玺[1] 陈立杰[1] 王媛媛[1] 王胜君[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110161

出　　处：《河南农业科学》2008年第5期72-75,共4页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金资助(30673199;30300231);国家农业科技成果转化基金资助(05EFN212100059);农业部科技成果转化基金资助(农计函[2004]110号)

摘　　要：木霉菌菌株Snef85是一株分离于土壤、对根结线虫有较高毒力作用的生防菌，在原液（1×），5×，10×，15×和20×稀释液浓度下，二龄幼虫的校正死亡率分别是98．12％，93．21％，59．56％，41．66％，13．85％。对其生物学特性进行研究，结果表明，该菌以PDA培养基生长最适；以葡萄糖为最佳碳源；以丙氨酸为最佳氮源；菌丝最适生长温度范围在25～30℃；最适pH为5．0～6．0；光照对菌丝生长和孢子着生影响不明显。Trichoderma harzianum was a biocontrol fungus that had more poisonous effect on Meloidogyne spp. The biological characters of strain Snef85 were studied. The results illustrated that the suitable cultural medium was PDA. The carbon and nitrogen sources for the growth were glucose and alanine. The optimum temperature and pH were 25--30℃ and 5.0--6.0, respectively. The illumination condition had no effect on the growth of Trichoderma spp.

关 键 词：根结线虫 生物防治 木霉菌 生物学特性 

分 类 号：S482.51[农业科学—农药学]