河南大尾寒羊血液蛋白多态性与多羔性的关系研究  

Relationship between Blood Protein Polymorphism and Prolificacy of Long-tail Han Sheep in Henan Province

作　　者：赵淑娟[1] 庞有志[1] 邓雯[1] 雷雪琴[1] 白俊艳[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003

出　　处：《河南农业科学》2008年第5期107-110,共4页Journal of Henan Agricultural Sciences

基　　金：河南省科技攻关项目(0124040025);河南科技大学科研基金项目(2003ZY15)

摘　　要：采用PAGE对河南大尾寒羊的血红蛋白(Hb)、转铁蛋白(Tf)进行多态性检测,同时以美利奴羊作为对照。结果表明:河南大尾寒羊Hb位点的HbB基因和HbBB基因型频率分别为0.694 5和0.500 0,且两品种差异显著,两群体中HbB基因均占优势。但大尾寒羊的HbB基因频率比美利奴羊群体高26.27%,HbBB基因型频率高66.7%,可能与其多羔性存在相关性。在36份河南大尾寒羊Tf血样中共检出5种基因(TfA,TfB,TfC,TfD,TfE)、10种表现型(TfAB,TfAC,TfAD,TfBB,TfBC,TfBD,TfCC,TfCD,TfCE,TfDD),TfCD产羔数最高为3个,TfCE的产羔数量最低为2个,不同表现型母羊的产羔数存在一定的差异。Taking Merion monotonous sheep as CK,the morphologism of Hb and TF of Long-tail Han Sheep in Henan province was tested by non-continuous vertical PAGE. The result showed that HbB gene and HbBB genotype frequence of Long-tail Han Sheep were 0. 694 5 and 0. 500 0, respectively,presenting significant difference between the two breeds （P〈0.05, P〈0.01）. The HbB genes in both multi-lamb Long-tail Han Sheep and single-lamb Merino monotocous sheep were in superiority,but the Hb gene frequence of Long-tail Han Sheep was 26.27% higher than that of Merino monotocous sheep（P〈0.01） ; HbBB genotype frequence of Long-tail Han Sheep is 66.7% higher than that of Merino monotocous sheep （P〈0. 01） ,which may relate to the multilamb feature. The Tf polymorphism test showed that among 36 bIoods of Long-tail Han Sheep, five genes （Tfa, TfB, TfC, TfD and TfE） and ten phenotypes （TfAB, TfAC, TfAD, TfBB, TfBC,TfBD, TfCC, TfCD, TfCE andTfDD） were detected. The highest lamb-breeding number is three from TfCD, and the lowest lamb-breeding number is two from TfCE. There existed some difference of lamb-breeding number between different phenotypes of female sheep,but not significant.

关 键 词：河南 大尾寒羊 血液蛋白多态性 基因频率 基因型频率 

分 类 号：S826.89[农业科学—畜牧学]