1,6-二磷酸果糖对犬体外循环中肺缺血-再灌注损伤保护作用的研究  

The protective effects of fructose-1,6-diphosphate on lung ischemia-reperfusion during cardiopulmonary bypass in dogs

作　　者：马彦敏[1] 方刚[1] 郭德和[1] 王云[1] 王虎[1] 杨剑[1] 杜学奎[1] 

机构地区：[1]宁夏医学院附属医院,宁夏银川750004

出　　处：《宁夏医学杂志》2008年第5期404-407,共4页Ningxia Medical Journal

摘　　要：目的探讨不同剂量1,6-二磷酸果糖(Fructose-1,6-d iphosphatin,FDP)对犬体外循环(Card iop-u lmonary Bypass)肺缺血-再灌注(Ischem ia-reperfusion,IR)损伤的作用效果和可能机制。方法建立犬体外循环IR损伤模型,将18只健康杂种犬随机分为三组:FDP大剂量组,FDP小剂量组,生理盐水对照组,每组各6只。在体外循环转机前(T1),阻断升主动脉30 m in(T2)、45 m in(T3),开放升主动脉45m in(T4),90m in(T5)检测各组肺组织Na+/K+-ATP酶活力、Ca2+/Mg2+-ATP酶活力超氧化物歧化酶(Superoxide d ismutase)活力、黄嘌呤氧化酶(xanth ine oxidase)活力、丙二醛(M alond ialdehyde)含量。体外循环转机前和结束时取右下肺组织测定肺湿/干比(W/D)值。结果(1)生理盐水对照组与FDP小剂量组肺组织比较:上述指标差别无显著性意义(P>0.05)。(2)生理盐水对照组和FDP大剂量组比较,肺组织SOD活力、Na+/K+-ATP酶活力和Ca2+/Mg2+TP酶活力水平均明显降低(P<0.05);肺组织肺湿/干重比值、XOD活力和MDA含量水平均明显增高(P<0.05)。(3)FDP大剂量组和FDP小剂量组比较:肺组织SOD活力、Na+/K+-ATP酶活力和Ca+/Mg2+-ATP酶活力水平均明显增高(P<0.05);肺组织肺湿/干重比值、XOD活力和MDA含量水平均明显降低(P<0.05)。结论(1)犬CPB中持续静滴大剂量1,6-二磷酸果糖(1 300mg/kg)可通过调节细胞ATP酶活力、SOD活力、XOD活力较大程度减轻肺组织缺血-再灌注损伤。(2)犬CPB中持续静滴小剂量1,6-二磷酸果糖(325mg/kg)对肺组织缺血-再灌注损伤无保护作用。Objective To investigate the effects of different dosage fructose - 1,6 - diphosphate on acute lung injury induced by lung ischemia - reperfusion during cardiopulmonary bypass in dogs and its possible mechanism. Methods After lung IR model during cardiopulmonary bypass in dogs was established, 18 adult mongrel canines （ 12 - 15kg）were randomly divided into 3 groups： large dosage FDP group（ infusion of FDP at a dose of 1300 mg/kg during cardiopulmonary bypass）, small dosage FDP group （infusion of FDP at a dose of 325 mg/kg during cardiopulmonary bypass） ,saline control group（ infusion of saline at a dose of 13ml/kg during cardiopulmonary bypass ）. The superoxide dismutase （SOD） activity, xanthine oxidase （ XOD ） activity, Na ^＋/K^＋ - ATPase activity, Ca^2＋/Mg^2＋ - ATPase activity, the levels of malondialdehyde（MDA） in lung tissue were examined before CPB （ Tl ） ,30min（ T2 ） and 45 min（ T3 ） after cross - clamping of aorta, and 45min（ T4 ） ,90min（ T5 ）. The wet/dry ratio of lung was analyzed at the end of CPB. Results （ 1 ） There was no significant difference between placebo control group and small dosage FDP group in all index（ P 〉 0. 05 ） ; （ 2 ） The activity of Na^＋/K^＋ - ATPase,Ca2^＋Mg^2＋ -ATPase,SOD in lung tissue of the placebo control group were all significantly lower than those of large dosage FDP group （P 〈 0. 05 ）. The wet/dry weight ratio of lung, the levels of MDA and XOD activity in lung tissue of large dosage FDP group were all significantly higher than those of placebo control group （ P 〈 0. 05 ）. （ 3 ） Comparison of variables between large dosage FDP group and small dosage FDP group ： the activity of Na^＋/K^＋ - ATPase ／ Ca^2＋ Mg^2＋ - ATPase, SOD in lung tissue of large dosage FDP group were all significantly higher than those of small dosage FDP group（ P 〈 0. 05 ）. The wet/dry weight ratio of lung tissue, the levels of MDA and XOD activity in lung tissue of large dosage FDP group were a

关 键 词：1 6-二磷酸果糖 犬 体外循环 缺血-再灌注 

分 类 号：R654.1[医药卫生—外科学]