中药对病毒性扩张型心肌病小鼠心肌ICAM-1的影响  

Effect of TCM on Expression of ICAM-1 in Cardiac Muscle on the Rat Models of Viral Dilated Cardiomyopathy

作　　者：周亚滨[1] 崔志勇[1] 赵艳霞[1] 张晶[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《辽宁中医杂志》2008年第5期676-678,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：黑龙江省杰出青年基金(JC0208)

摘　　要：目的:通过观察益气养阴、活血化瘀方对病毒性扩张型心肌病小鼠心肌ICAM-1表达水平的影响。方法:210只Balb/c小鼠随机分为4组。通过反复多次腹腔注射柯萨奇B3m病毒,建立病毒性扩张型心肌病小鼠模型。中药低剂量组、中药高剂量组用益气养阴、活血化瘀方治疗。于实验终点观测各组小鼠心肌病理形态学改变、死亡率、体重、心脏质量指数变化,SABC法检测小鼠心肌ICAM-1表达水平。结果:中药低剂量组、中药高剂量组小鼠心肌ICAM-1表达水平显著低于模型组(P<0.01),且心肌病理改变亦较模型组轻微。结论:益气养阴、活血化瘀方可下调病毒性扩张型心肌病小鼠心肌ICAM-1表达水平,减轻炎性细胞损伤和自身免疫损伤。Objective ：To investigate the effect of the prescription ofyiqi yangyin huoxue huayu on the rat models of viral dilated cardiomyopathy by observing the expression of ICAM - 1 in cardiac muscle. Methods ： Replicated the animal models of the viral dilated cardiomyopathy with the method of injecting the CVB3m virus to the peritoneal cavity of the Balb/c rats. 210 Balb/c rats were devided into 4 groups at random,ie, blank group, model group, low dose traditional Chinese medicine group and high dose traditional Chinese medicine group. The blank group has 30 rats. The other three group has 60 rats each. Treat the rats of low dose traditional Chinese medicine group and high dose traditional Chinese medicine group with the prescription of yiqi yangyin huoxue huayu. Observe the change of pathomorphology in the cardiac muscle tissue , the rate of death ,the weight change and the cardiac weight index in the end of the experiment. At the same time SABC method was used to exam the expression of ICAM- 1 in cardiac muscle. Results ： Compaired with the model group the expression of ICAM - 1 in cardiac muscle was significantly lower and the change of pathomorphology in the cardiac muscle tissue were lighter in the low dose traditional Chinese medicine group and high dose traditional Chinese medicine group. Conclusions： The prescription ofyiqi yangyin huoxue huayu can reduce the the expression of ICAM - 1 in cardiac muscle and relieve the damage of inflammatory reaction and autoimmunity in DCM.

关 键 词：病毒性扩张型心肌病 益气养阴 活血化瘀方 柯萨奇病毒B3M 细胞间黏附因子-1 

分 类 号：R259.422[医药卫生—中西医结合]