去势兔骨质疏松模型建立的初探  

Primary Study on the Use of Ovariectomized Rabbits for Establishment of Osteoporosis Model

作　　者：杨霞[1] 蔡桂英[1] 魏玲[1] 候静 李炯[1] 郑虎[2] 翁玲玲[2] 

机构地区：[1]华西医科大学生物化学教研室,成都610041 [2]华西医科大学药化教研室,成都610041

出　　处：《生物医学工程学杂志》1997年第4期353-358,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金

摘　　要：选用兔作模型进行探索，以期证明兔原发性骨质疏松动物模型建立的可行性。通过切除双侧卵巢的方法诱发兔骨质疏松动物模型的建立，术后5～8个月内观察其骨密度、体重、毛色及生长状况，处死后取骨作生物力学测定，病理组织学检查，烧灰测灰重及灰的钙、磷含量，观察其子宫重．结果表明：骨密度、弯曲强度、灰重及灰的钙、磷含量、子宫重均明显降低（P＜0．05），骨小梁数目减少，皮质变薄．综合以上各项指标，兔骨质疏松模型已初步建立。本研究为探明原发性骨质疏松发病机理及评价防治药物疗效的研究提供重要的基础．Purebred New Zealand female rabbits were used in this study for demonstrating the feasibility of es-tablishing rabbit's primary osteopirosis model. Bilateral ovariectomies were performed. Five to eight months after operation,we observed the BMD,weight,hair colour and living condition of the model. After killing the rabbits,we made biomechanical detection and pathologic histomorphometry using the bones. The weight of bone ash,the con-tent of calcium and phosphorus,and the weight of metra were determined. The results showed that BMD, bone bending stress,weight of bone ash,content of calcium and pkosphorus in ash,and weight of metra all decreased (P <0.05);the number of trabecula decreased and the bone cortex became thinner. All these parameters evidenced the primary founding of rabbit's osteoporosis model. This research provides a basis for studying the pathogenesis of primary osteoporosis and evaluating the efficacy of pharmacotherapy.

关 键 词：原发性 骨质疏松 去势兔模型 动物模型 

分 类 号：R681.02[医药卫生—骨科学]