高效液相色谱-蒸发光散射法测定红药片中三七皂苷R1及人参皂苷Rg1、Re、Rb1的含量  

Determination of notoginsenoside R_1,ginsenoside Rg_1,Re and Rb_1 in Hongyao tablets by HPLC-ELSD

作　　者：段瑞[1] 沙东旭[1] 杨文 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023 [2]葫芦岛市药品检验所,辽宁葫芦岛125000

出　　处：《沈阳药科大学学报》2008年第5期376-380,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的 用高效液相色谱-蒸发光散射（HPLC-ELSD）法测定红药片中三七皂苷R1及人参皂苷Rg1、Re、Rb1的含量。方法 色谱柱填料为氨基键合硅胶（4．6mm×250mm，5μm），流动相为乙腈-水（体积比为80：20），漂移管温度为90℃，载气流速为2．IL·min^-1。结果 三七皂苷R．及人参皂苷Rg1、Re、Rb1质量分别在0．214～2．14μg、0．808～8．08μg、0．3084～3．04μg及0．996～9．96μg内的对数值与相应峰面积的对数值呈良好线性关系；制剂中4种成分的平均回收率（n=6）分别为96．7％（RSD=2．1％）、96．0％（RSD=1．2％）、96．8％（RSD=2．4％）和96．6％（RSD=2．5％）。结论 该方法准确、分离效果好、无干扰，可用于红药片的质量控制。Objective To establish the quantitative method of notoginsenoside R1, ginsenoside Rg1, Re and Rb1 in Hongyao tablets （traditional Chinese medicine）by HPLC-ELSD. Methods The column used was a Lichrospher NH2（4.6 mm ×250 mm） packed with a 5 μm stationary phase. The mobile phase consisted of acetonitrile-water（ V： V = 80：20）. The temperature of drift tube was 90 ℃ and the nebulizer nitrogen flow rate was 2.1 L·min^-1. Results The linear relationship were obtained within the range of 0.214 - 2.14 μg, 0. 808 - 8.08 μg, 0. 308 4 - 3.04 μg and 0. 996 - 9.96 μg for the four components respectively. The average recoveries of the four components（ n = 6） were 96.7 % （RSD = 2.1% ）, 96.0 % （RSD = 1.2 % ）, 96.8 % （RSD = 2.4 % ） and 96.6 % （ RSD = 2.5 % ）, respectively. Conclusions It is proved that the HPLC-ELSD method is reliable, well separated, and precise; it can be used in quantitative analysis of Hongyao tablets.

关 键 词：红药片 三七皂苷R1 人参皂苷RG1 人参皂苷Re人参皂苷Rb1 高效液相色谱-蒸发光散射检测法 

分 类 号：R917[医药卫生—药物分析学]