弓形虫抗原的部分理化性质研究  

Research of Partial Physicochemical Quality of Toxoplasma Antigen

作　　者：林爱芬[1] 傅翠娥[1] 余毅[1] 杨旭明[1] 张乐[1] 陈彩华[1] 

机构地区：[1]浙江省医学科学院,杭州310013

出　　处：《中国人兽共患病杂志》1997年第6期20-22,共3页Chinese Journal of Zoonoses

基　　金：浙江省自然基金

摘　　要：本文选用RH株弓形虫速落子细胞质、细胞膜和腹水代谢抗原，观察温度、pH值对抗原活性的影响，并分别测定糖及蛋白质含量。结果表明，三种抗原对热处理都较为稳定，但代谢抗原在加热前后的检测效果均不及其它两者。经糖含量测定显示，代谢抗原的糖含量最低。结果提示，弓形虫抗原的重要决定因子可能是糖蛋白或多糖成分。pH值试验表明，细胞质抗原的包被波以pH9．6碳酸盐缓冲液为佳，而细胞膜抗原应选用pH6.5磷酸盐缓冲液。We chose the cytoplasm,cell membrane and metabolic antigen of toxoplasma RH strain to observe the anti-genic activity affected by heat and pH and determine the contents of saccaride and protein.The results showed that three antigens treated by heat were all stable.The diagnostic effect of matabolic antigen was inferior to the other two.Determination of saccharide content showed that saccharide in metabolic antigen was the least of three antigens.The results implied that the glycoprotein or polysaccharide might be the most important determinating factor of toxoplasma antigen.pH test showed that pH9.6 carbonate buffer was better for cytoplasmic antigen while pH6.5 phosphate buffer was better cell membrane antigen.

关 键 词：弓形虫 抗原 理化性质 

分 类 号：R531.8[医药卫生—内科学] R382.5[医药卫生—临床医学]