去细胞异体神经移植后T细胞亚群及细胞内细胞因子的实验研究  

T lymphocyte subsets and intracellular cytokines after transfer of chemical acellular nerve allograft

作　　者：李威[1] 衷鸿宾[2] 林星石[3] 侯树勋[2] 吴闻文[2] 阮狄克[1] 

机构地区：[1]北京海军总医院骨科,100037 [2]解放军总医院第一附属医院骨科 [3]解放军总医院免疫教研室

出　　处：《中华创伤骨科杂志》2008年第5期450-454,共5页Chinese Journal of Orthopaedic Trauma

基　　金：首都医学发展科研基金（2002-3049）

摘　　要：目的为化学去细胞同种异体周围神经移植的临床应用提供进一步的免疫学实验依据。方法128只BALB／C小鼠分随机分为假手术组、自体神经移植组、新鲜异体神经移植组和化学去细胞异体神经移植组，每组32只。对各实验组分别进行相应的手术。分别在术后3、7、14、28d将各组8只小鼠脾淋巴细胞进行分离，特异性荧光标记的单克隆抗体作用后，经流式细胞仪检测T淋巴细胞亚群及细胞内细胞因子的水平及其变化趋势。结果在各时间点中，化学去细胞神异体经移植组CD3^＋、CD4^＋、CD8^＋、CD25^＋阳性细胞率以及IL-2、IFN-1、TNF-α阳性细胞率与假手术组、自体神经移植组相比差异均无统计学意义（P〉0．05）。而新鲜异体神经移植组与其他三组比较差异有统计学意义（P〈0.05）。结论化学去细胞处理的同种异体神经的免疫源性等于或接近于自体神经，明显低于新鲜同种异体神经。Objective To provide immunologieal evidence for elinieal transfer of ehemieal extraeted acellular nerve allograft Methods One hundred and twenty-eight BALB/C mice were randomly divided into 4 groups of equal size according to their different treatments： negative contrast group （NC）, fresh autograft group （AG）, fresh allogeneic nerve group （FN） and chemical extracted acellular allogeneic nerve group（CEN）. Then we implanted various kinds of nerve grafts into the thigh muscle of BALB/C mice in corresponding groups. At 3, 7, 14, 28 days postoperatively, 8 mice from each group were killed each time to harvest their spleens, from which T lymphocytes were collected. Then monoclonal antibodies （CD3, CIM CD8 CD25, IL-2, IFN-γ, TNF-α） were added into the suspension. Then fluorescence-activated cell sorting （FACS） was used to determine the positive rates of cells combined with the above monoclonal antibodies. Results There were no statistically significant differences between CEN group, NC group, and AG group, but indexes of FN group were significantly higher than those of the other 3 groups at corresponding time points. Conclusion There is no obvious immune rejection of chemical extracted acellular nerve aUograft when compared with fresh nerve autograft.

关 键 词：周围神经 神经移植 免疫 T细胞亚群 细胞因子 

分 类 号：R651.3[医药卫生—外科学]