水稻耐1，2，4-三氯苯胁迫基因型的苗期筛选  

Screening of Tolerant Rice Genotypes to 1,2,4-Trichlorobenzene Stress at Seedling Stage

作　　者：陈文军[1] 张国良[1] 孙国荣[1] 戴其根[1] 张洪程[1] 陶金飞[2] 孙洁[2] 严林锋[2] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,江苏扬州225009 [2]淮阴工学院生命科学与化学工程学院,江苏淮安223001

出　　处：《农业环境科学学报》2008年第3期1003-1008,共6页Journal of Agro-Environment Science

基　　金：国家科技攻关计划重大项目(2004BA520A03);国家科技支撑计划重大项目(2006BAD02A03);江苏省高校自然科学基础研究面上项目(07KJD180018)

摘　　要：为了评定不同水稻基因型对1,2,4-三氯苯(TCB)的耐性强弱,确定合适的筛选指标和筛选浓度,在人工气候箱条件下,采用发芽试验和砂培水稻幼苗的方法,对幼苗期水稻基因型耐TCB胁迫的差异性进行了鉴定,并确定了水稻基因型耐TGB胁迫合适的筛选指标和筛选环境。结果表明,以0.35mmol·kg-1沙浓度TCB更适合用于水稻基因型的耐性鉴定。以发芽势、发芽率、最长根长、根数、株高、地上部干重、根干重和总干重的性状相对值(处理测定值/对照测定值×100%)作为幼苗耐性指数(tolerance index,TI),其中相对最长根长、相对株高、相对地上部分干重、相对根干重和总干重是评价水稻对TCB胁迫耐性强弱的较好指标。基于耐性指数对各基因型进行聚类分析,将50个水稻基因型聚为耐性、较耐、较敏感和敏感4类。1,2,4-trichlorobenzene （1,2,4-TCB） has pervaded in natural environment and industrial production. It would cause a series of problems clue to its longevity and amassment. The EPA has added it into the environmental pollutant list. In order to evaluate the tolerance of the fifty rice genotypes to 1,2,4-TCB stress and identify proper screening indices and concentration, the effects of 1,2,4-TCB on the rice seed germination and seedling were studied. At first, the concentrations of 1,2,4-TCB treated 20 genotypes were 0 mmol·kg^-1 sand, 0.35 mmol·kg^-1 sand and 3 mmol·kg^-1 sand, and 1,2,4-TCB dissolved in 0.3 mL acetone were added into sands. The result of this experiment showed that 0.35 mmol·kg^-1 sand was the better screening concentration. So 0 mmol·kg^-1 sand and 0.35mmol·kg^-1 sand were adopted to treat 50 genotypes in the latter study. Germination potential, germination rate, the longest root length, root number, plant height, the dry weight of shoot and roots of different rice varieties at seedling stage were measured. Character relative values were got from measure values of treated groups and control groups. They were used as tolerance indices （TI） of early seedlings. Cluster analysis of different wheat varieties based on TI of the longest root length, plant height, the dry weight of shoot and roots was adopted. The results showed that the fifty rice varieties could be grouped into four types, .i.e. TCB sensitive varieties, relatively sensitive varieties, tolerant varieties and relatively tolerant varieties.

关 键 词：水稻 基因型 TCB胁迫 耐性评定 

分 类 号：Q945.78[生物学—植物学]