Megsin基因E1-5’UTR区A267G与免疫球蛋白A型肾病阴虚证的相关性  

Correlation between traditional Chinese medicine syndromes in primary immunoglobulin A nephropathy and A267G in 5'-untranslated region within exonal of megsin gene

作　　者：钟逸斐[1] 陈以平[1] 邓跃毅[1] 陈楠[2] 王朝晖[2] 

机构地区：[1]上海中医药大学龙华医院肾内科,上海200032 [2]上海交通大学医学院瑞金医院肾内科,上海200025

出　　处：《中西医结合学报》2008年第5期463-467,共5页Journal of Chinese Integrative Medicine

摘　　要：目的:观察增生为主的原发性免疫球蛋白A型肾病(immunoglobulin Anephropathy,IgAN)患者阴虚证(气阴两虚证、肝肾阴虚证)与megsin基因E1-5’UTR区A267G的相关性,寻找原发性IgAN中医证型微观辨证可能的物质基础。方法:筛选符合标准的原发性IgAN患者120例,进行megsin基因E1-5’UTR区A267G的单核苷酸多态性(single nucleotide polymorphism,SNP)测序,观察中医证型与SNP的相关性。结果:120例患者中GG型83例,GA型34例,AA型3例。肝肾阴虚证在AA与GA型IgAN患者中所占比例较高,气阴两虚在GG型中所占比例较高(P<0.01);GG型与GA型+AA型比数比(odds ratio,OR)为9.800,95%可信区间(confidence interval,CI)为3.969～24.199。这样的差异同样存在于不同性别和不同年龄之间。结论:Megsin基因E1-5’UTR区A267G可能是区分原发性IgAN肝肾阴虚证和气阴两虚证的物质基础之一。Objective： To observe the correlation between traditional Chinese medicine （TCM） syndromes （＂ deficiency of qi and yin＂ and ＂deficiency of liver yin and kidney yin＂ ） and A267G in 5＇-untranslated region within exonal of megsin gene, and to search the substantial genetic basis for micro-differentiation of TCM syndromes in primary immunoglobulin A nephropathy （IgAN）. Methods： A total of 120 IgAN cases meeting the diagnostic criteria were enrolled. The sequence of single nucleotide polymorphism （SNP） of A267G in 5＇-untranslated region within exonal of megsin gene was tested. The correlation between SNP and TCM syndromes was observed. Results： There were 83 cases carrying GG genotype, 34 cases carrying GA genotype and 3 cases carrying AA genotype in 120 cases of primary IgAN. There was a high proportion of ＂deficiency of liver yin and kidney yin＂ in IgAN cases with AA and GA genotypes, and a high proportion of ＂deficiency of qi and yin＂ in IgAN cases with GG genotype （P〈0.01）. Odds ratio in TCM syndrome distribution between GG genotype and GA plus AA genotype was 9. 800, and 95% confidence interval was 3. 969-24. 199. The discrepancy also resided in IgAN patients with different genders and ages. Conclusion： A267G in 5＇-untranslated region within exonal of the megsin gene may be one of the substantial genetic basis for differentiating ＂deficiency of liver yin and kidney yin＂ syndrome and ＂deficiency of qi and yin＂ syndrome in primary IgAN.

关 键 词：免疫球蛋白A型肾病 多态性 单核苷酸 基因 MEGSIN 

分 类 号：R277.5[医药卫生—中医学]