幽门螺杆菌球形体回复原形的实验研究  

Study on Coccoid Forms of Helicobacter pylori Possible Recovery in Vitro and in Vivo

作　　者：曾浩[1] 郭刚[1] 毛旭虎[1] 童文德[1] 邹全明[1] 

机构地区：[1]第三军医大学临床微生物学及免疫学教研室,重庆400038

出　　处：《中国比较医学杂志》2008年第5期32-36,I0006,共6页Chinese Journal of Comparative Medicine

基　　金：国家863计划资助课题(编号2003AA215020);国家"九五"重点科技攻关项目(编号96-901-01-54)

摘　　要：目的对幽门螺杆菌(Helicobacter pylori,Hp)球形体进行体内、外回复原形的比较研究,揭示其潜在的传播途径。方法在布氏肉汤的基础上设计了4种Hp再生培养基,对Hp螺旋体、原生质体及球形体进行体外培养;同时采用30只蒙古沙土鼠(Mongolian gerbil)进行体内感染定植实验,对感染小鼠胃粘膜进行Hp定量培养和组织学检测。结果Hp球形体在4种再生培养基中均未能回复生长;而在感染小鼠的体内却观察到了Hp球形体的回复定植。Hp螺旋体感染组在小鼠胃内的定植密度较高,且胃粘膜下可见大量炎症细胞浸润;而球形体感染组并未见到小鼠胃粘膜组织的明显炎症损伤,且仅有少量回复的螺旋体定植。结论Hp球形体作为一种低水平代谢休眠体,代谢活性及毒力均有所减弱,但仍具有潜在的致病性。本研究支持部分Hp球形体具有活力但体外不能培养成活这一假说,提示"粪-口"传播应引起更多的关注。Objective To study coccoid forms of Helicobacter pylori （Hp） possible recovery in vitro and in vivo, reveal its potential mute of transmission. Methods On the basis of Brucella broth, four kinds of regenerated culture medium were designed and make them approach intragastfic physiological enviroment. In addition, thirty Mongolian gerbils also used. coccoid forms of Hp were inoculated in four culture mediums as well as animals and the possible recovery was observed in vitro and vivo. Results The recovery and regrowth in vitro of coccoid forms of Hp did not be observed in our applied four kinds of culture medium. Parts of coccoid forms of Hp occured reversion and colonization in mice stomach. But the level of colonization and pathological change of the group of coccoid forms were significantly lower compared with the group of spiral forms. Conclusions This experiment results suggested coccoid forms of Hp still possess potential pathogenicity, and provides an impetus for this hypothesis that coccoid forms of Hp be viable but nonculturable.

关 键 词：幽门螺杆菌 球形体 螺旋体 蒙古沙土鼠 模型 

分 类 号：R379.9[医药卫生—病原生物学]