广西壮、汉族哮喘儿童人类白细胞抗原-B、-DR基因的多态性  

Study on Human Leukocyte Antigen-B and-DR Alleles Polymorphism in Children with Asthma in Zhuang and Han Nationality in Guangxi

作　　者：谢庆玲[1] 秦岭[2] 焦伟[3] 甄宏[1] 王琳[4] 刘晓春[3] 胡琼燕[1] 谭颖[1] 潘红飞[1] 黄文珊[5] 陆元奉[1] 杜华[1] 

机构地区：[1]广西壮族自治区人民医院儿科,南宁530021 [2]广西壮族自治区人民医院神经内科,南宁530021 [3]广西壮族自治区人民医院检验科,南宁530021 [4]广西壮族自治区人民医院遗传室,南宁530021 [5]广西壮族自治区人民医院呼吸内科,南宁530021

出　　处：《实用儿科临床杂志》2008年第4期253-256,共4页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(3026004/C011005);广西壮族自治区自然科学基金项目资助(桂科自0339027)

摘　　要：目的探讨广西壮、汉族人类白细胞抗原（HLA）-B、-DR位点的基因多态性与儿童哮喘发病的关联性。方法将3代居住广西南宁地区无血缘关系的84例哮喘儿童（汉族57例，壮族27例）和168例无哮喘和特应性疾病的健康人（汉族83例，壮族85例）纳入研究。所有哮喘儿童应用强生法玛西亚系统检测其血清总IgE水平、10种吸入性变应原皮肤点刺试验和肺功能检测。利用基因芯片法检测HLA-B的40个和HLA-DR的26个等位基因。分别计算汉族和壮族人群中哮喘和健康对照组基因频率，进行χ^2检验，分别比较组间基因频率差异和危险性分析，计算基因与哮喘的关联强度比值比（OR值）。结果HLA-B位点共检出27个等位基因，HLA-DR检出21个等位基因。HLA-B46、HLA-DRB1＊070X和HLA-DRB1＊11XX等位基因仅在汉族的哮喘与健康人群分布有差异，哮喘与健康对照组比较有显著差异（Pa〈0．05），OR值（95％CI）分别为0．388（0．153～0．983）、7．885（0．896～69．399）和2．782（1．188～6．516），且HLA-DR B1＊070X等位基因在本组壮族人群中未见出现。壮族人群中，HLA-B56、-B61和-DRB1＊1001等位基因在哮喘与健康对照组间有分布差异，哮喘与健康对照组比较有显著差异，组间HLA-B56经，检验有统计学意义（χ^2=6．588P〈0．01），HLA-DRB1＊1001经Fisher精确概率法检验有统计学意义（P=0．043）；HLA-B56和HLA-DRB1＊1001的OR值（95％C1）分别为9．432（1．713～51．932）和10．50（1．044～105．590）。而HLA-B61等位基因在壮族哮喘患者中未见出现。结论HLA-B和HLA-DR的基因多态性在壮、汉族间分布有差异；HLA—B56和HLA—DRB1＊1001等位基因可能为壮族人群的哮喘易感基因；而汉族人群中HLA-DRB1＊070X和HLA-DRB1＊11XX等位基因是易感基因，HLA-B46等位基因可能为保护基因。Objective To explore the association between the characteristics of the genetic polymorphism of human leukocyte antigen （HLA） - B and HLA - DR alleles and asthma in Guangxi Zhuang and Han nationality children. Methods Eighty - four blood - unrelated asthmatic individuals ,57 cases of Han nationality and 27 cases of Zhuang children with asthma, and 168 healthy controls, included 83 cases of Han nationality and 85 cases of Zhuang people without asthma and atopy living in Nanning region of Guangxi were involved in the study. All asthmatic patients＇serum total IgE levels were measured with UniCAP Pharmacia system, and skin -prick test with ten kinds of inhalant aller- gens were taken and pulmonary functions were measured among the patients. HLA oligonucleotide array was used to 40 gene alleles of HLA - B and 26 HLA - DR. Comparison of the frequency distribution of HLA - DR alleles and HLA - B alleles in 2 groups was evaluated by chi - square test, and the risk for asthma of the HLA allele＇s carriers was valuated by OR value. Results Twenty - seven alleles at HLA - B locus and 21 alleles at HLA - DR were detected. The distribution of HLA - B46, HLA - DRB1 ＊ 070X and HLA - DRB1 ＊ llXX alleles only in Han people was distinctive in the asthmatic patients compared with those in healthy controls（ P 〈 0.05 ）. OR were 0. 388 （95% CI：0. 153 - 0. 983 ） ,7. 885 （95% CI：0. 896 - 69. 399 ） and 2. 782 （95 % CI： 1. 188 - 6. 516 ）, respectively, while HLA - DR B1 ＊ 070X allele was failed to be detected in the Zhuang nationality. Among the Zhuang nationality people, the distribution of HLA - B56 allele, HLA - B61 allele and HLA - DRBI ＊ 1001 allele were distinctive in the asthmatic patients compared with those in healthy controls, HLA - B56 allele （X2 = 6. 588 P 〈 0.01 ） and HLA - DRB1 ＊ 1001 allele P = 0. 043 （ Fisher precise probabilistic method）, respectively. OR of HLA - B56 allele was 9. 432 （ 95 % CI： 1.713 - 51. 932） , HLA - DRB1 ＊ 1001 allele was 10.50 （ 95 % C1：

关 键 词：壮族 汉族 哮喘 人类白细胞抗原 广西 

分 类 号：R725.6[医药卫生—儿科]