白萝卜提取物促胃动力作用机制  

Kinetogenic effects mechanism of Ecr in gastrointestinal tract

作　　者：王伟[1] 王景杰[1] 颜云龙[1] 夏德雨[1] 刘美媛[1] 张晓兰[1] 黄裕新[1] 

机构地区：[1]第四军医大学唐都医院消化科,陕西西安710038

出　　处：《胃肠病学和肝病学杂志》2008年第5期378-379,共2页Chinese Journal of Gastroenterology and Hepatology

基　　金：陕西省科技攻关课题(2004K10-G4(1))

摘　　要：目的探讨白萝卜提取物(Raphanus sativus L extract,Ecr)促胃肠动力作用机制。方法20只SD大鼠随机分为A(Ecr)组、B(0·9%氯化钠对照)组,分别灌服Ecr和等体积0·9%氯化钠,1h后,免疫组织化学方法和放免法分别观察胃电起搏区P物质(SP)、降钙素基因相关肽(CGRP)表达的变化。结果SP免疫阳性神经及产物的表达明显增加(P<0·05);CGRP免疫阳性神经及产物的表达明显减少(P<0·05)。结论白萝卜提取物的促胃肠动力作用可能与局部及血浆中SP、CGRP量的变化有密切关系。Objective To study the kinetogenic effect mechanism of Raphanus sativus L extract（Ecr）. Methods Twenty SD rats were randomly divided into A（Ecr） group and B（control）group. Ecr and 0.9% NaCL were given respectively. The distributions of SP and CGRP in gastric body were investigated by immunohistochemistry assay and by using the radioimmunoassay method. The values of SP and CGRP in the blood plasma were measured and compared with each other before and after reperfusion 1 h. Results The expressions of SP increased significantly （P 〈 0.01 ）. The expressions of CGRP decreased significantly （P 〈 0.05） in gastric body of rats. Conclusion The kinetogenic effect of Ecr is closely correlated to the increase of SP expression and the decrease of CGRP.

关 键 词：白萝卜提取物 P物质 降钙素基因相关肽 免疫组织化学方法 

分 类 号：R333.2[医药卫生—人体生理学]