微分脉冲伏安法用于血液中两种核苷类抗病毒药物的同时测定  

Differential Pulse Voltammetric Determination of Two Antivirus Nucleoside Analogues in Blood of Rat

作　　者：张阅[1] 倪永年[1] 

机构地区：[1]南昌大学化学系,南昌330047

出　　处：《分析化学》2008年第5期668-672,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20365002,20562009);江西省自然科学基金(No.0620041);中国科学院长春应用化学研究所电分析化学重点实验室(No.SKLEAC2004-3);长江学者及创新团队项目(No.IRT0540)资助项目

摘　　要：采用微分脉冲伏安法考察了两种核苷类抗病毒药物阿昔洛韦（aciclovir）和喷昔洛韦（penciclovir）在乙二胺修饰的玻碳电极上的电化学行为及其在代谢血样中的同时测定方法，并探讨了电极修饰和电极反应的机理。在pH2．56的Britton-Robinson缓冲溶液中，阿昔洛韦和喷昔洛韦在乙二胺修饰的玻碳电极上均有-灵敏的不可逆氧化峰，峰电位分别为1．20和1．17V。在优化的实验条件下，阿昔洛韦和喷昔洛韦分别在0．20-4．0mg／L和0．02-0．40mg／L浓度范围内与峰电流呈线性关系，其检出限分别为77和12μg／L。引入化学计量学方法对其混合组分的伏安谱进行解析，实现了两组分的同时测定，并对小鼠血液中代谢的两种核苷类抗病毒药物进行了同时测定。Differential pulse voltammetry was applied to the simultaneous determination of two antivirus nucleoside analogues, aciclovir and penciclovir, in the rat metabolic blood at an ethylenediamine modified glassy carbon electrode. Moreover, the mechanism of the electrode modification and the electrode reaction was investigated and discussed. In the pH 2.56 Britton-Robinson buffer, both aciclovir and penciclovir had a sensitive irreversible oxidation peak on the modified glassy carbon electrode. The peak potentials were 1.20 V and 1.17 V for these two medicines, respectively. Under the optimal conditions, the peak current was linear with its concentration in the range of 0. 20 - 4.0 mg/L for aciclovir and 0.02 - 0.40 mg/L for penciclovir. The detection limits were 77 μg/L for aciclovir and 12 μg/L for penciclovir. Furthermore, chemometrics of classical least squares （ CLS）, principal component regression （PCR） and partial least squares （PLS） were applied to resolve the overlap spectra of the admixture of the two medicines, without troublesome procedures of separation and purification. The proposed method has been applied to the determination of the two antivirus nucleoside analogues in the metabolic blood of mouse.

关 键 词：阿昔洛韦 喷昔洛韦 化学修饰电极 化学计量学 微分脉冲伏安法 核苷类抗毒药物 

分 类 号：R96[医药卫生—药理学]