液相色谱-串联质谱法测定饲料中三聚氰胺残留  

Determination of Melamine Residues in Feed by Liquid Chromatography Tandem Mass Spectrometry

作　　者：李爱军[1] 张代辉[1] 马书民[1] 李明[1] 张秀珍 姚天灵 

机构地区：[1]吉林出入境检验检疫局,长春130062 [2]长春大成玉米开发有限公司,长春130062

出　　处：《分析化学》2008年第5期699-701,共3页Chinese Journal of Analytical Chemistry

基　　金：国家科技部重点课题资助项目(No.2002EE550021)

摘　　要：应用液相色谱-串联质谱法测定饲料中三聚氰胺残留。试样用V（乙腈）：V（H2O）=1：1溶液，提取，高速离心后，供液相色谱一串联质谱仪定性定量分析。流动相为V（乙腈）：V（H：O）=80：20混合溶液。采用电喷雾离子源，定性离子对为127．2／85．2和127．2／68．2；定量离子对为127．2／85．2。在添加了0．5～10．0mg／kg的三聚氰胺标准品时的回收率为92．6％～103．2％；相对标准偏差（RSD）在0．8％～2．0％；检出限为0．2mg／kg。A sensitive method was developed for the determination of melamine residues in feed by liquid chromatography-tandem mass spectrometry （LC/MS/MS）. Test sample is extracted with water-acetonitrile （1：1, V/V）. The extract is centrifuged and the supematant is determined and confirmed by LC/MS/MS. Mobile phase in LC is water-acetonitrile （80： 20, V/V）. Electro-spray ionization source is applied. The determination ion pairs are 127.2/85.2 and 127.2/68.2, and the confirmation ion pairs are 127.2/85.2. While the spiked contents of melamine standard are 0. 5 - 10. 0 92.6% - 103.2%, the RSDs are in 0.8% - 2.0%. The limit mg/kg , the recoveries are in the range of of determination is 0.2 mg/kg.

关 键 词：液相色谱-串联质谱 三聚氰胺 饲料 

分 类 号：S816[农业科学—饲料科学]