栽培绿豆V2709抗豆象特性遗传及基因初步定位  

Heredity Analysis and Gene Mapping of Bruchid Resistance of a Mungbean Cultivar V2709

作　　者：孙蕾[1] 程须珍[1] 王素华[1] 王丽侠[1] 刘长友[1] 梅丽[1] 徐宁[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081

出　　处：《中国农业科学》2008年第5期1291-1296,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30170635);作物种质资源保护项目(NB04-22-12);国家科技基础条件平台项目(2004DKA30380-08);国家科技支持计划(2006BAD02B08);农业行业科技专项(nyhyzx07-017)

摘　　要：【目的】绿豆[Vigna radiata(L.)Wilczek]是豇豆属亚洲亚属(Ceratotropis)中的一个栽培豆种,豆象是危害绿豆的重要害虫,利用分子标记对抗豆象基因进行初步定位,为该材料在绿豆育种中的利用和抗豆象基因的分离奠定基础。【方法】利用原产于印度的抗豆象栽培绿豆V2709与农艺性状优良的感豆象推广品种中绿1号(VC1973A)配制杂交组合,对杂种F2、BC1F1和F3进行遗传分析;采用BSA法,以上述F2代为定位群体,利用抗、感亲本和抗、感池筛选63个RAPD引物和113对SSR/STS引物。【结果】遗传分析结果表明该抗豆象特性由一对显性基因控制;引物筛选结果表明2个引物能在抗、感池间扩增出多态性条带,用Mapmaker EXP3.0b软件作图,将该抗豆象基因初步定位在一个RAPD标记和一个STS标记之间,遗传距离分别为11.0cM和5.8 cM,并暂定名为Br2。【结论】本研究定位的基因标记可作为绿豆抗豆象育种分子标记辅助选择的一种工具。[Objective] Mungbean [Vigna radiata （L.） Wilczek] belongs to Ceratotropis. Bruchid is a main pest of mungbean and can bring great harm to mungbean. Molecular markers for Bruchid-resistant are to be identified for mungbean breeding and Bruchid-resistant gene obtaining. [Method] By using V2709, a mungbean cultivar with bruchid resistance from India, and Zhonglv No.1 （VC1973A）, a bruchid susceptible variety form AVRDC as parents. At the same time, 63 RAPD markers, 113 SSR/STS primers were used for BSA analysis to find molecular marker that link with the resistant gene. [Result] The segregation of bruchid resistance in the F2 and BC1F1 and F3 populations showed that the bruchid resistance of V2709 is controlled by one dominant gene. As the results, OPC-06 and STSbr2 were identified to be related with the bruchid resistant gene. After verified in F2 individuals, the genetic distances between these two markers and the target gene were 11.0 cM and 5.8 cM respectively. The bruchid gene of V2709 was called Br2. [Conclusion] The identified molecular markers related to the Mungbean Bruchid-resistant gene in this study will benefit for marker-assisted selection in breeding programs.

关 键 词：绿豆V2709 抗豆象 遗传分析 STS RAPD 

分 类 号：S522[农业科学—作物学]