23个小麦抗叶锈病近等基因系SRAP多态性  

Molecular Diversity of 23 Wheat Leaf Rust Resistance Near-Isogenic Lines Determined by Sequence-Related Amplified Polymorphism

作　　者：刘雅辉[1] 闫红飞[1] 杨文香[1] 李星[1] 李亚宁[1] 孟庆芳[1] 张立荣[1] 刘大群[1] 张汀[1] 

机构地区：[1]河北农业大学植物病理系/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001

出　　处：《中国农业科学》2008年第5期1333-1340,共8页Scientia Agricultura Sinica

基　　金：高校博士基金(20050086002);国家"973"前期项目(2005CCA01600)

摘　　要：【目的】分析以Thatcher为遗传背景的小麦抗叶锈病近等基因系的遗传多样性,探讨SRAP技术在小麦抗叶锈病基因标记及基因克隆研究中应用的可行性。【方法】采用SRAP(sequence-related amplified polymorphism)技术对23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系和感病对照Thatcher进行分析。【结果】从128对引物中筛选得到41对具有多态性引物,每个引物组合产生6～41个多态性条带,共产生537个多态性条带,多态性条带率达49.5%,平均每个引物组合产生13.1个多态性条带;每个引物组合产生1～13个特异性条带,共产生115个特异性条带,特异性条带率为10.6%,平均每个引物组合产生2.8个特异性条带。聚类分析将23个小麦抗叶锈病近等基因系和Thatcher在相似系数0.72处分为A、B两大类,其中96%的个体归入B类。B类又分为Ⅰ、Ⅱ两个亚类,95%的个体聚在第Ⅱ亚类。第Ⅱ亚类在相似系数为0.785附近分为4小类。【结论】23个小麦抗叶锈病近等基因系间存在一定的差异。SRAP技术是一种简单有效的分子标记系统,可在小麦叶锈病抗病基因的研究中应用。[Objective] The research analysed the genetic diversity of wheat leaf rust resistance near-isogenic lines in Thatcher background and investigated the feasibility of SRAP marker applied in study of wheat leaf rust resistance genes marker and clone. [Method] SRAP （sequence-related amplified polymorphism） technique was used in analysis of twenty-three wheat leaf rust resistance near-isogenic lines and Thatcher. [ Result] Forty-one of 128 SRAP primer combinations identified from a total of 537 reproducible fragments. Among them, 49.5% were polymorphic, and 115 of them were specific bands （10.6%）. Six to 41 fragments were amplified per primer combination, with an average of 13.1 bands. The number of polymorphic fragments for each primer combination varied from 1 to 13, with an average of 2.8. A cluster analysis was performed using NTSYSpc Version 2.10e. Cluster analysis revealed twenty three wheat leaf rust resistance near-isogenic lines and Thatcher into two clusters of A and B with the similarity coefficient of 0.72. 96% of them were clustered into cluster B, which was divided into groupⅠ and group Ⅱ. 95% of the near isogenic lines in cluster B fell into group II. Group II included four small groups with 0.785 similarity coefficient. [ Conclusion ] It is concluded that the twenty-three wheat leaf rust resistance near-isogenic lines are different at molecular level. All these results showed SRAP marker is simple, economic and effective, and it could be used in study of wheat leaf rust resistance genes.

关 键 词：小麦抗叶锈病基因 近等基因系 SRAP 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]