美洲斑潜蝇不同寄主种群及地理种群间的β-tubulin基因序列分析  

Differentiation of Different Host-Populations and Geo-Populations of Liriomyza sativae Blanchard Based on β-tubulin Gene Sequences

作　　者：杜予州[1] 王莉萍[1] 陆亚娟[1] 郑福山[1] 陆自强[1] 

机构地区：[1]扬州大学应用昆虫研究所,江苏扬州225009

出　　处：《中国农业科学》2008年第5期1542-1547,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30370932);国家“973”项目(2006CB102002);江苏省科技攻关项目(BE2001343)

摘　　要：【目的】通过对美洲斑潜蝇β-tubulin基因序列的比较,分析不同寄主种群和地理种群的遗传分化情况。【方法】对中国美洲斑潜蝇Liriomyza sativae Blanchard 5个寄主种群以及6个地理种群的β-微管蛋白基因(β-tubulingene)进行测序,运用软件DNAStar和MEGA对美洲斑潜蝇不同地理种群及寄主种群的β-tubulin基因序列的遗传分歧及相似性进行分析,并建立系统发生树。【结果】DNAStar和MEGA两种软件对美洲斑潜蝇不同寄主种群以及地理种群间的遗传关系的分析可得到相似的结果。在美洲斑潜蝇寄主种群和地理种群序列变异分析中,共发现8个变异位点,序列长度比较保守,均没有碱基的插入或缺失。美洲斑潜蝇不同寄主种群之间以及地理种群之间的β-tubulin基因序列相似性极高,都在98%以上。【结论】虽然基于β-tubulin基因序列所显示的美洲斑潜蝇各寄主种群之间以及地理种群之间的遗传分化很小,但是其分化趋势分别与对寄主的嗜好程度以及地理分布基本吻合。[ Objective ] To analyze the genetic differentiation of the host- and geo-populations of Liriomyza sativae Blanchard. [ Method ] The β-tubulin gene of 5 host-populations and 6 geo-populations of L. sativae were sequenced. The identity and variation of β-tubulin gene sequenced were analyzed by biosoβware DNAStar and MEGA and the phylogenetic trees were constructed. [ Result ] The results obtained by the biosoftware DNAStar and MEGA respectively were similar. The differences of β-tubulin gene among the host- and geo-populations of L. sativae were very low, with 8 variable sites and no insertions and deletions and the identities were all above 98%. [ Conclusion ] The trends of differentiation of host- and geo-populations of L. sativae were consistent with the hobby to hosts and the geographical distribution, respectively.

关 键 词：美洲斑潜蝇 寄主种群 地理种群 β-tubulin基因 遗传分化 

分 类 号：S433[农业科学—农业昆虫与害虫防治]