南阳牛HCRTR1基因编码区单核苷酸多态性分析  

Single Nucleotide Polymorphisms (SNPs) in Hypocretin Receptor 1 (HCRTR1) Gene of Nanyang Cattle

作　　者：张丽[1] 张爱玲[1] 张良志[1] 张春雷[1] 朱泽轶[1] 雷初朝[1] 王新庄 陈宏[1,2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西杨凌712100 [2]徐州师范大学细胞与分子生物学研究所,江苏徐州221116 [3]河南省肉牛工程技术研究中心,郑州450003

出　　处：《中国农业科学》2008年第5期1560-1566,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30771544);国家“863”计划项目(2006AA10Z197);国家支撑计划(2006BAD01A10-5);河南省杰出人才创新基金项目(0521001900);西北农林科技大学拔尖人才支持计划项目

摘　　要：【目的】研究南阳牛HCRTR1基因编码区单核苷酸多态性,为利用遗传标记进行肉牛选育奠定基础。【方法】以122头南阳牛为试验动物,利用PCR-SSCP及测序方法研究了HCRTR1基因全部编码区序列的多态性,并对发现的SNP位点进行了遗传特性及单倍型分析。【结果】在该基因的编码区首次发现11个SNP位点,分别为G322A、G384C、T420C、C423T、T481A、C510A、T627C、C631T、G690A、G714A和C736T,其中在322、481、631和736bp处的突变都导致了氨基酸的改变,而其余7处均为同义突变。在322、510和736bp处的SNP表现为中度多态,PIC分别为0.3731、0.3190和0.2851,其余8处的SNP位点均呈现低度多态。对多态位点进行单倍型分析,发现在南阳牛122个个体中共检测到29种单倍型,其中有2种单倍型的频率超过了10%;7种单倍型的频率小于1%;其余20种单倍型的频率均在1%～10%。【结论】本研究测定了南阳牛122个个体的HCRTR1基因的多态性,在全部编码区检测到11个多态性单核酸变异,构成了29种单倍型。[Objective] To analyze the single nucleotide polymorphisms （SNPs） in hypocretin receptor 1 （HCRTR1） gene of Nanyang cattle. [Method] One hundred and twenty two Nanyang cattle were used for SNPs discovery in the complete coding region of HCRTR1 gene using PCR-SSCP and sequencing methods. Haplotypes and genotypes were reconstructed and analyzed. [Result] Eleven SNPs at G322A, G384C, T420C, C423T, T481A, C510A, T627C, C63 IT, G690A, G714A and C736T were detected. The variations at 322, 481, 631 and 736 bp caused amino acid replacements and variations at the remaining seven SNPs were synonymous. Polymorphism information content （PIC）, number of effective alleles （Ne） and heterozygosity （h） were also estimated for the 11 SNPs. Three SNPs （322 bp, 510 bp and 736 bp） were moderate polymorphic with a PIC of 0.3731, 0.3190 and 0.2851, respectively, while the remaining eight SNPs had low polymorphisms （〈0.25）. In 122 Nanyang cattle, 29 haplotypes were reconstructed. The frequencies of two haplotypes were more than 10%, of seven haplotypes less than 1% and of the remaining 20 haplotypes＇ between 1%-10%. [Conclusion] Eleven SNPs and 29 haplotypes were discovered in the complete coding region of HCRTR1 gene in 122 Nanyang cattle.

关 键 词：南阳牛 HCRTR1基因 单核苷酸多态性 单倍型 

分 类 号：S823[农业科学—畜牧学]