高效液相色谱间接光度检测法测定心脏停搏液中钠、钾、钙、镁的含量  

Determination of sodium, kalium, magesium and calcium in liquor of cardioplegia by high performance liquor chromatography-indirect photothetric determination method

作　　者：袁成[1] 王景祥[1] 梁竹[1] 焦素云[1] 李凡东[1] 

机构地区：[1]济南军区总医院,济南250031

出　　处：《药学实践杂志》1997年第4期234-237,共4页Journal of Pharmaceutical Practice

摘　　要：本文用高效液相色谱间接光度检测法测定了心脏停搏液中钠、钾、钙、镁的含量。以SoherisorbSCX为固定相，0．35mmol／L硫酸铈的[水-乙腈（85：15），并用磷酸调pH至3．2～3．4]溶液为流动相，亚铈离子作为流动相平衡离子直接应用光度检测器测定，并与其他测定方法比较。结果表明，分离度较好，104％＞回收率＞97％，线性关系良好。结果与滴定法相比无显著性差异。该法具有操作简便快速，灵敏准确，适于作为制剂中4种离子的常规检测方法。To determine the content of sodium,kalium, magnesium and calcium in liquor of cardioplegia by HPLC-IPD method.METHOD: The chromatographic system consisted of Soherisorb SCX column and mobile phase of the solution of 0.35 mmol/L cerium sulphate in water-acetonitrile(85: 15), that were regulated to pH 3.2~3. 4 with phosphoric acid. Ceritnn was a balance - ion in mobile phase, therefore the content of sodium, kalium, magnesium and calcium were determined directly by photometer. The detected wave length was UV254 um. RESULTS:The mean recovery of 4 ion were between in 97~104% and deteCtion limits of sodium,kalium,magnesium and calcium were 5, 8, 5 and 4 ng. There was noremarkable betWeen in this HPLC-IPD method and titrative method. CONCLUSION: The method is constant, sensitive, have a concentration and is good for determination of sodium, kalium, magnesium and calcium concentration.

关 键 词：心脏停搏液 钠 钾 钙 镁 高效液相色谱法 

分 类 号：R972[医药卫生—药品] R927.2[医药卫生—药学]