一个抑制AP-1活性的人类新基因AC3-33的克隆和初步功能研究  

Molecular cloning and preliminary function study of a novel human gene AC3-33 related to suppress AP-1 activity

作　　者：刘鹏[1] 邓唯唯[2] 高鹏[2] 陆阳[2] 孙博[1] 李明[1] 赵杰[1] 石太平[2] 张秀军[1] 

机构地区：[1]华北煤炭医学院生物科学系,唐山063000 [2]国家人类基因组北方研究中心,北京100176

出　　处：《遗传》2008年第5期575-582,共8页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30671092)资助~~

摘　　要：Activator protein-1(AP-1)是重要的转录因子,其活性失调与肿瘤等多种疾病直接相关。本文运用"高通量高内涵细胞筛选技术(high throughput-high content cell-based screening technology)"对650个以未知功能基因为主的人类基因进行AP-1双荧光素酶报告基因筛选(Dual-Luciferase reporter gene screening),获得了一个可抑制佛波酯(PMA)加离子霉素(Inonmycin)诱导的AP-1活性的人类新基因AC3-33(GenBank中该基因名为C3orf33,No.FLJ31139)。生物信息学分析该基因序列全长1931bp,由6个外显子和5个内含子组成,定位于3q25.31,从271～1026有一个编码251个氨基酸的可读框,编码一个约29kDa的蛋白,在肾上腺和宫颈等多种组织都有表达。AC3-33与其他人类已知蛋白质没有明显的同源性,亚细胞定位于细胞质中,许多氨基酸序列高度保守。初步实验结果显示AC3-33是一个有重要功能的人类新基因。Activator protein-1 （AP-1） is an important transcription factor, and dysregulation of its activity has been associated with many human diseases, including various cancers. A novel human gene AC3-33 （GenBank name： c30rf33, Accession No. FLJ31139）, which can suppress PMA and Ionomycin induced activation of AP-1, was identified from 650 human function-known genes by using the high throughput-high content cell-based screening technology. The gene whose full cDNA length is 1391 bp containing 6 exons and 5 introns is located in the human chromosome 3q25.31. The predicted pro- tein encoded by this gene contains 251 amino acids with a theoretical molecular weight of 29 kDa. Expression of the AC3-33 gene is widly found in adrenal glands and cervix. The amino acid sequences of AC3-33 is highly conserved, and has no homology to other known proteins. Subcellular localization studies show that the AC3-33 protein was localized in the cytoplasm. Our preliminary results showed that AC3-33 is an important novel gene related to supress AP- 1 activity.

关 键 词：AP-1 基因克隆 双荧光素酶报告基因筛选 AC3-33 

分 类 号：Q78[生物学—分子生物学]