猪脂肪组织中IGF2和IGFBP3基因表达的发育性变化及其品种差异  

Developmental expression changes of IGF2 and IGFBP3 genes in adipose tissue of two pig breeds

作　　者：郭玉姣[1] 唐国庆[1] 李学伟[1] 朱砺[1] 李明洲[1] 

机构地区：[1]四川农业大学动物科技学院,雅安625014

出　　处：《遗传》2008年第5期602-606,共5页Hereditas（Beijing)

基　　金：教育部长江学者和创新团队发展计划项目(编号:IRT0555-6);教育部高等学校博士学科点专项基金项目(编号:20060626003)资助~~

摘　　要：采用荧光定量PCR技术分析长白猪和太湖猪背部皮下脂肪组织中胰岛素样生长因子2和胰岛素样生长因子结合蛋白3基因在30、60、90、120和150日龄时表达水平的发育性变化。结果表明:(1)品种内日龄间比较,长白猪IGF2mRNA在30日龄时的表达量极显著高于其他日龄(P<0.01),之后逐渐下降,至120日龄降到最低,150日龄时又明显上升;太湖猪IGF2mRNA在30～60日龄的表达量较高,90日龄降至最低,120日龄迅速回升,之后又有所下降。两品种IGFBP3mRNA表达的发育性变化模式基本相同,30日龄时表达量最高,60日龄显著下降(P<0.05),之后趋于平缓但略有波动。(2)品种间同日龄比较,120日龄时太湖猪IGF2mRNA的表达量极显著高于长白猪(P<0.01),150日龄时太湖猪IGFBP3的表达量极显著低于长白猪(P<0.01),其余日龄间两品种差异均不显著(P>0.05)。研究结果提示:猪脂肪组织IGF2和IGFBP3基因表达存在明显的发育性变化和品种差异;IGF2基因表达水平的降低可能与脂肪细胞的增殖有关。To investigate the developmental expression patterns of Insulin-like Growth Factor 2 （IGF2） and Insulin-like Growth Factor Binding protein 3 （IGFBP3） genes at the age of 30, 60, 90, 120 and 150 days in back subcutaneous fat tissue of Landrace and Taihu pigs, real-time fluorescence quantitative PCR was applied. Results showed that the expression level of IGF2 mRNA in Landrace pigs was significantly higher at 30 days than other days （P〈0.01）, then gradually declined and lowest at 120 days, obviously increased at 150 days. In Taihu pigs, it was relatively higher at 30-60 days, lowest at 90 days, increased rapidly at 120 days, and then decreased. Developmental expression patterns of IGFBP3 mRNA in the two pig breeds were similar, which were highest at 30 days, declined significantly at 60 days （P〈0.05） then maintained steady, but a little fluctuant between ages in each breed. In addition, the expression level of IGF2 mRNA in Taihu pigs was significantly higher than that of Landrace pigs at the age of 120 days （P〈0.01）, and the expression level of IGFBP3 mRNA in Taihu pigs was significantly lower than that of Landrace at the age of 150 days （P〈0.01）, with no significant difference between the two pig breeds at other ages （P〉0.05）. The results suggest that the expression of IGF2 and IGFBP3 mRNA in the adipose tissue of pigs exhibits specific developmental changes and different patterns between the two pig breeds. The expression decrease of IGF2 may be related to adipocyte hyperplasia.

关 键 词：猪 脂肪组织 荧光实时定量PCR 胰岛素样生长因子2(IGF2) 胰岛素样生长因子结合蛋白3(IGFBP3) 

分 类 号：S828[农业科学—畜牧学] Q953[农业科学—畜牧兽医]