心肌梗死后心力衰竭大鼠模型的构建及细胞因子变化  

Establishing of rat heart failure model in myocardial infarction： the changes of cytokines

作　　者：李彦华[1] 王士雯[1] 赵栋[1] 

机构地区：[1]解放军总医院老年心血管病研究所,北京100853

出　　处：《中国综合临床》2008年第5期404-406,共3页Clinical Medicine of China

基　　金：国家重点基础研究发展规划项目“973课题”（G2000057004）

摘　　要：目的构建大鼠的心力衰竭模型，检测心肌梗死后不同时期血清细胞因子的表达情况，探讨在心力衰竭的发生过程中细胞因子的演变。方法结扎冠状动脉前降支的方法构建大鼠的心肌梗死后心力衰竭模型，在术前及术后4、16周分别进行心脏超声检查及血流动力学监测，对心功能进行评定，并检测相应血清中TNF-α、IL-6的浓度。结果①假手术组应用ELISA法未检测到血清中存在上述两种细胞因子，心力衰竭代偿期TNF-α、IL--6的血清浓度均升高，在心力衰竭失代偿期，尽管仍能检测到TNF-α，但血清水平成明显下降趋势；此时IL-6的血清浓度进一步升高；②在16周时，大鼠的心脏超声与血流动力学检测具有较高的诊断符合率，大鼠心脏超声作为一种无创性的检测手段，可以用于评定大鼠的心脏功能状态。结论①细胞因子的持续升高与心肌梗死后心脏功能的恶化有关，不同的细胞因子浓度变化存在不同的时相，TNF-α的升高早于IL-6，呈一过性增高趋势，后者在体内尤其是血清中增高的持续时间较长，表达在心力衰竭的代偿及失代偿期都升高，但失代偿期增高更明显，IL-6的血清细胞因子浓度与心功能损害的关系更大。②在大鼠的心力衰竭模型构建过程中，心脏超声可以替代血流动力学标准用于心力衰竭模型构建过程中的监测。Objective To establish rat heart failure（HF） model following myocardial infarction（MI） and then detect the possible cytokines expression levels at different HF groups, so to explore the development of cytokines levels during the progression of heart failure. Methods The model was established by ligature of the left descending coronary artery. The rats Were assessed by echocardiography and cardiac catheterization before and after 4 and 16 weeks following coronary ligature. Serum levels of TNF-α and IL-6 were determined. Results ①Serum cytokines could not be detectable in sham group using ELISA Methods, while in operated group, the concentration of TNF-α and IL-6 were significantly higher, especially IL-6. ②The diagnosis coincidence between echocardiography and hemodynamics was high. Echocardiography can be used as an invasive measure to assess cardiac function in rat heart failure models. Conclusion ①Cytokines content is higher in compensated compared with the other two groups, especially to the IL-6 expression. ②Cardiac ultrasound can be used as a useful method in monitoring postinfarction heart failure rat model, especially as an invasive measure. It can provide us successive data of the model.

关 键 词：心力衰竭 细胞因子 白细胞介素-6 肿瘤坏死因子-Α 

分 类 号：R542.22[医药卫生—心血管疾病] R541.6[医药卫生—内科学]