脱氢野百合碱诱发肺动脉高压犬的循环内皮祖细胞数量和功能变化  

Quantitative and functional changes of circulating endothelial progenitor cells in dogs with dehydromonocrotaline-induced pulmonary artery hypertension

作　　者：曾春来[2] 夏良[1] 马彩艳[1] 刘善宽[1] 胡晓晟[1] 王兴祥[1] 陈君柱[1] 

机构地区：[1]浙江大学医学院附属第一医院心内科,杭州310003 [2]浙江省丽水市中心医院

出　　处：《中华结核和呼吸杂志》2008年第5期330-334,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的 观察脱氢野百合碱（DHMC）诱发犬肺动脉高压形成前后循环内皮祖细胞数量和功能的变化。方法10只Beagle犬经右心室注射DHMC诱导肺动脉高压（PAH）。注射DHMC前、注射后6周采集静脉血，用流式细胞仪分析AC133和KDR检测双阳性的细胞数量。收集单个核细胞体外培养7d后进行乙酰化低密度脂蛋白胆固醇（DiLDL）摄取和凝集素-I（UEA-I）结合反应，并进行体外血管生成试验。计量资料采用孟x±s表示，采用配对t检验进行统计学分析。结果 10只Beagle犬注射DHMC后9只存活，1只于第2天死亡。注射DHMC后6周肺动脉平均压由（11．3±2．0）mm Hg（1 mm Hg=0．133 kPa）增高到（20．2±1．6）mm Hg（t=10．307，P〈0．01）。PAH形成前后经流式细胞仪分析的AC133和KDR双阳性细胞数量分别为（632．8±42．8）个／ml和（206．1±26．8）个／ml（t=25．361，P〈0．01）；体外培养7d的细胞中UEA-I和DiLDL染色双阳性细胞数量分别为（41±6）个／200倍视野和（22±6）个／200倍视野（t=6．510，P〈0．01）。体外成血管试验中形成的血管数为（21．1±2．8）支／200倍视野和（11．2±2．8）支／200倍视野（t=7．583，P〈0．01）。结论 犬肺动脉高压形成后循环内皮祖细胞数量减少，成血管能力下降。Objective To detemfine the quantitive and functional changes of circulating endothelial progenitor cells （EPCs） in dogs with dehydromonocrotaline-induced pulmonary artery hypertension （PAH）. Methods Dehydromonocrotaline was injected into the canine right ventricle to induce pulmonary hypertension. Circulating EPCs were enumerated as AC^＋133, KDR^＋ cells by fluorescence-activated cell sorter using counting beads, and the number and activity of EPCs after in vitro expansion were determined by acLDL uptake/lectin staining assay and in vitro tubule forming assay. Results Nine of the 10 beagles survived after dehydromonocrotaline injection. Six weeks later, mean pulmonary artery pressure increased from （11.3 ±2.0） mm Hg（1 mm Hg= 0. 133 kPa） to （20.2±1.6） mm Hg （t =10.307, P〈0.01）, and the AC^＋133 and KDR^＋ cells decreased from （632. 8 ±42. 8） cells/ml to （206. 1±26. 8） cells/ml （t = 25. 361, P 〈0. 01 ）. UEA-I and DiLDL positive cells deceased from （41±6） EPC8/×200 field to （22±6） EPCs/×200 field （t =6. 510, P 〈0. 01 ）. In addition, in vasculogenesis assay, PAH EPCs formed less quantitative （ 11.2 ± 2. 8 vs 21.1 ± 2. 8 tubules/×200 field, respectively, t = 7. 583, P 〈 0. 01 ） and less qualitive tubules than baseline EPCs. Conclusion The number and vessel forming ability of EPCs are impaired in this canine model of dehydromonocrotaline-induced pulmonary hypertension.

关 键 词：干细胞 内皮 高血压 肺性 野百合碱 

分 类 号：R686[医药卫生—骨科学]