芍药苷对小鼠巨噬细胞产生TGF-β1的影响  

Effect of Paeoniflorin on Secretion of TGF-β1 from Macrophages in Mice

作　　者：储德勇[1] 李丛磊[1] 李静[1] 罗飞[1] 郑美娟[1] 吴强[2] 罗庆礼[1] 沈继龙[1] 

机构地区：[1]安徽医科大学病原生物学教研室,教育部省部共建和安徽省重要遗传病基因资源利用重点实验室,人畜共患病安徽省重点实验室,合肥230032 [2]安徽医科大学病理学教研室,合肥230032

出　　处：《中国寄生虫学与寄生虫病杂志》2008年第2期81-85,共5页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.30571631)~~

摘　　要：目的探讨芍药苷(paeoniflorin,PAE)对血吸虫可溶性虫卵抗原(SEA)刺激小鼠腹腔巨噬细胞(PMs)产生转化生长因子β1(TGF-β1)的影响。方法研磨法制备SEA,分别加入含有小鼠PMs的培养板、培养瓶及培养皿中,培养24h,ELISA法测定TGF-β1含量,RT-PCR检测PMs内TGF-β1基因的表达,Western blotting检测PMs内TGF-β1蛋白的表达;在含PMs的培养瓶和培养皿中分别加入10mg/LSEA5ml,培养12h,再分别加入不同浓度的PAE(0、7.5、15、30、60及120mg/L),继续培养12h和24h。RT-PCR与蛋白质印迹(Westernblotting)分别检测PAE对SEA刺激的PMs内TGF-β1基因与蛋白的表达。结果10mg/L的SEA可明显刺激PMs产生TGF-β1(235.86±3.43ng/L),PAE呈浓度依赖性地抑制TGF-β1mRNA的表达(r=-0.827,P<0.01)和TGF-β1蛋白的表达(r=-0.952,P<0.01)。结论PAE能抑制SEA刺激的PMs产生TGF-β1。Objective To explore the effect of paeoniflorin （PAE） on the production of transforming growth factor β1 （TGF-β1） from peritoneal macrophages（PMs）stimulated by soluble egg antigen （SEA）of Schistosoma japonicum. Methods SEA was prepared by trituration and added into culture plank, flask and dish containing PMs which were cultured for 24 h. TGF-β1 secreted from PMs was measured by ELISA. TGF-β1 mRNA and protein produced from PMs were evaluated by RT-PCR and Western blotting, respectively. SEA （10 mg/L） 5 ml was added into culture flask and dish containing PMs. PMs were cultured for 12 h, and PAE at different concentrations （0, 7.5, 15, 30, 60, 120 mg/L） was added into the culture flask and dish, and PMs were cultured consecutively for another 12 h and 24 h, respectively. TGF-β1 mRNA and protein from PMs stimulated by SEA were evaluated by RT-PCR and Western blotting, respectively. Results TGF-β1 （235.86±3.43 ng/L） was produced from PMs under stimulation of SEA at 10 mg/L, and the expression of TGF-β1 mRNA and protein in PMs were depressed significantly by PAE in a concentration-dependent manner （t=-0.827, P〈0.01; r=-0.952, P〈0.01, respectively）. Conclusion PAE inhibits the production of TGF-β1 from PMs stimulated by SEA.

关 键 词：芍药苷 日本血吸虫 虫卵可溶性抗原 肝纤维化 转化生长因子Β1 巨噬细胞 

分 类 号：R383.24[医药卫生—医学寄生虫学]