p16^INK4a蛋白在宫颈疾病中的表达特点及其与HPV感染的关系  

Expression of p16^(INK4a) protein and its relationship with HPV in cervical cancer and precancerous lesions

作　　者：李军果[1] 李力[1] 郭建新[1] 高奉浔[2] 赵雯[3] 

机构地区：[1]第三军医大学大坪医院野战外科研究所妇产科,重庆 400042 [2]第三军医大学大坪医院野战外科研究所病理科,重庆 400042 [3]西南医院病理学研究所,重庆 400038

出　　处：《第三军医大学学报》2008年第10期928-931,共4页Journal of Third Military Medical University

基　　金：重庆市科委自然科学基金(2006BB5431);重庆市计划生育科研项目(20060103)~~

摘　　要：目的研究伴有高危型人乳头瘤病毒(high-risk human papillomavirus,hr-HPV)感染及无hr-HPV感染的宫颈癌(cervical cancer,CC)、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及宫颈炎中p16INK4a蛋白表达水平的变化及意义,探讨宫颈癌及癌前病变中p16INK4a蛋白表达与HPV感染的相关性。方法应用免疫组化方法检测126例宫颈疾病(CC40例,CIN67例和宫颈炎19例)组织中p16INK4a蛋白的表达,其中,hr-HPV(+)宫颈疾病89例(CC32例,CIN48例和宫颈炎9例),hr-HPV(-)宫颈疾病37例(CC8例,CIN19例和宫颈炎10例),进行半定量分析及统计学检验。结果40例CC中,p16INK4a全部阳性表达;48例hr-HPV(+)和19例hr-HPV(-)CIN中,p16INK4a阳性表达分别为39例和12例;9例hr-HPV(+)和10例hr-HPV(-)宫颈炎组织中,p16INK4a阳性表达均为4例。hr-HPV(+)或hr-HPV(-)宫颈炎、宫颈上皮内瘤变和宫颈癌组织中p16INK4a蛋白表达水平均依次升高。CC和CIN中p16INK4a蛋白表达与hr-HPV感染相互间无显著相关性(P>0.05)。结论细胞周期抑制蛋白p16INK4a在CC及癌前病变中过表达,且与hr-HPV感染无关,提示p16INK4a蛋白在宫颈癌的发生、发展中所起的作用和作用机制可能与其在其他恶性肿瘤中不同。检测p16INK4a蛋白表达有助于CC的早期诊断。Objective To study whether p16^INK4a protein expressions in cervical cancer （CC）, cervical in- traepithelial neoplasia （CIN） and cervicitis are associated with high-risk human papillomavirus （hr-HPV） infection. Methods The expressions of p16^INK4a protein were detected in 126 samples of cervical lesions （40 CC, 67 CIN, 19 cervicitis） by immunohistoehemical staining, among which 89 cervical lesions were hr-HPV（ ＋ ） （32 CC, 48 CIN, 9 cervicitis） and 37 hr-HPV（-） （8 CC, 19 CIN, 10 eervicitis）. Results p16^INK4a protein was positive in all of CC samples. The positive expression of p16^INK4a was in 39 hr-HPV （ ＋ ） samples and 12 hr-HPV（-） CIN samples, with no significant difference between each other. The positive expression of hr-HPV infection. Pearson correlation analysis revealed no correlation between hr-HPV infection and p16^INK4a protein expressions in CC and CIN （ P 〉 0. 05）. Gonclusion Overexpression of INK4a gene indicates the potential of CC development. Moreover, there is no correlation between the overexpression of p16^INK4a protein and hr-HPV state in cervical lesions. Therefore, it is likely that p16^INK4a protein expression is not induced bv the same carcinogenic mechanisms of CC and other malignant tumors.Overexpression of NIK4a gene helps in the diagnosis of cervical cancer.

关 键 词：宫颈癌 免疫组织化学 HPV 蛋白 P16^INK4A 

分 类 号：R373[医药卫生—病原生物学] R730.23[医药卫生—基础医学]