地塞米松血管内支架的涂层制备及体外缓释实验研究  

Coating for dexamethasone-eluting intravascular stent and its drug sustained release in vitro

作　　者：陈勇鹏[1] 王贵学[2] 陈琰[3] 晋献春[1] 侯延斌[2] 罗来龙[2] 孙大贵[4] 

机构地区：[1]第三军医大学新桥医院中医科 [2]重庆大学生物工程学院 [3]第三军医大学新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室,重庆400037 [4]重庆大学化学化工学院,重庆400044

出　　处：《第三军医大学学报》2008年第10期935-937,共3页Journal of Third Military Medical University

基　　金：国际科技合作重点项目(2004DFA06400);第三军医大学中青年基金(06XG030);重庆市科技攻关项目(2006AA5014-3);重庆市发改委产业化前期项目(2005-1006)~~

摘　　要：目的制备地塞米松血管内支架的涂层,观察体外缓释特性。方法采用喷涂、浸涂两种方法制备支架,以高效液相色谱仪检测载药量,并进行体外药物缓释测定。结果喷涂支架的载药量为(24.26±5.23)μg,浸涂4d的载药量为(93.15±7.83)μg。浸涂4d的支架,药物缓慢释放,15d时释放84%。结论制备的支架具有明显的缓释作用。Objective To prepare the coating of dexamethasone-eluting intravascular stent and observe the features of its delayed release in vitro. Methods Spray painting and dipping coating were used respectively to prepare stent coating, and then high performance liquid chromatography （HPLC） was used to detect the drug loading. The stents prepared by dipping coating were set in a flow of 50 ml PBS buffer at a velocity of 20 ml/min, and 0.5 ml buffer was collected at 6 h and 1,2, 4, 6, 8, 10, 12, 15 d respectively with another 0.5 ml fresh PBS being added immediately. The drug content of the 0.5 ml buffer was detected by HPLC for drug sustained release in vitro. Results The drug loading of stents prepared by spray painting was （24.26 ± 5.23）μg, while that stents prepared by dipping coating for 4 d was （93.15 ±7.83） μg.Drugs were released slowly and the release rate reached 84% at 15 d. Conclusion The dipping coating-prepared stents have evi- dent effect of sustained release.

关 键 词：地塞米松 支架 体外研究 缓释 

分 类 号：R944.9[医药卫生—药剂学] R969[医药卫生—药学]