冬小麦抗旱种质资源遗传多样性的RAPD分析  

RAPD analysis of genetic diversity of winter wheat germplasm resourses with drought resistance

作　　者：王淑英[1] 樊廷录[2] 张金文[1] 

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070 [2]甘肃省农业科学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2008年第2期41-45,共5页Journal of Gansu Agricultural University

基　　金：黄土高原土壤侵蚀与旱地农业国家重点实验室基金:黄土高原旱地冬小麦抗旱节水快速诊断指标研究课题(10501-162);“十一五”国家科技支撑计划:西北半湿润偏旱区粮果稳产高效技术集成研究与示范(2006BAD29B07)

摘　　要：采用RAPD技术对42份冬小麦抗旱种质资源进行了遗传多样性的研究.从100个引物中筛选出了22个有效引物,共扩增出221条DNA带.应用SPSS10.0分析软件对供试材料间RAPD标记欧氏遗传距离,并按Av-erage Linkage法进行了聚类分析.结果显示:42份材料可明显分为6类,第Ⅰ类有32份材料,其中国内材料21份、国外材料11份,分为A、B 2个亚类6个组;其余10份国外材料分属5个类,R2独立构成第Ⅲ类,并最后与其他各类合并,R2与各材料的遗传差异性最大.The genetic diversity of 42 winter wheat genetypes with drought resistance was analysed by RAPD. Twenty two available primers were screened from 100 primers, and 221 DNA bands were amplified. Based on squared euclidean distance, the 42 materials were clustered into six groups by average linkage method. Group Ⅰ including 21 materials from USA and 11 materials from China, which could be divided into section A and B. The rest 10 materials from USA were clustered into other five groups. R2 composed group Ⅲ alone,and had the biggest genetic difference with other materials.

关 键 词：冬小麦 种质资源 遗传多样性 RAPD 

分 类 号：S512.1[农业科学—作物学] Q78[生物学—分子生物学]