水杨酸诱导的甜瓜DDRT-PCR分析及其基因差异表达片段的分离  

Isolation of gene fragment with differential expression in melon induced by SA using DDRT-PCR technique

作　　者：方春媛[1] 陈年来[1] 任晓燕[1] 张涛[1] 荆世杰[1] 

机构地区：[1]甘肃农业大学资源与环境学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2008年第2期90-95,共6页Journal of Gansu Agricultural University

基　　金：甘肃省农业生物技术专项经费资助(GNSW-2006-06).

摘　　要：利用DDRT-PCR技术,比较了1 mmol/L SA诱导第4天与未经SA诱导的甜瓜感白粉病品种卡拉克赛的基因表达差异.共获得差别条带19条,在SA诱导后出现差异带15条,在未诱导的对照中出现差异带4条.对其中的8条差异片段回收、克隆,用EcoRI/HindIII双酶切和PCR扩增.对其中的cDNA3-4-1测序,在BLAST上比较同源性,发现其与拟南芥热激转录因子hsf8基因具有83%的核苷酸同源性.说明1 mmol/L SA处理的甜瓜叶片在mRNA水平上发生了明显的变化,SA诱导了热激转录因子hsf8基因的大量表达,从而可能影响热激蛋白hsp70的表达,对维持蛋白质结构,稳定胞内环境具有积极作用.In this paper, it investigated the variation of resistance-related genes induced by SA using mRNA Differential with 1 mmol/L SA. Display technology. Melon （Cucumis melo. L. cv. Kalakese）with 3 leaves was treated After 4 days of treatment, cDNA were used in mRNA differential display. Altogether 19 differential bands were observed, among these bands indicated that cDNA3-4-1 had the 83 differential bands were cloned. Sequencing results of identity with the putative heat shock factor protein （hsf8） mRNA. Results showed that there was significant in mRNA level with SA treatment,and may affect hsp70 expression which played an important role in maintain protein structure, stability intracellular environment.

关 键 词：甜瓜 SA MRNA差别显示 hsf8 

分 类 号：S652[农业科学—果树学] Q786[农业科学—园艺学]