虫草素钌(Ⅱ)配合物的合成及其对大鼠肝癌细胞杀伤作用的初步研究  

The Synthesis of A Novel Ruthenium (Ⅱ) Complex with Cordycepin and Preliminary Research of Its Killing Action on Rat Liver Cancer Cells

作　　者：许琳[1] 刘艺[1] 谭宇蕙[2] 梅文杰[3] 

机构地区：[1]广州中医药大学中药学院,2004级本科生广东广州510006 [2]广州中医药大学基础医学院,广东广州510006 [3]广东药学院药科学院无机化学教研室,广东广州510006

出　　处：《广州中医药大学学报》2008年第3期215-219,共5页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】探讨虫草素钌(Ⅱ)配合物的合成及其对大鼠肝癌细胞株CBRH7919的杀伤作用。【方法】以虫草素(Cordycepin)为主要配体,联吡啶(2′,2′-bipyridine)为辅助配体合成钌配合物,将产品初步分离提纯后,以空白组、虫草素组、钌前体组、虫草素与钌前体等摩尔浓度混合的混合物组作为对照组,各组均设0.2、1.0、5.0、25.0、125.0 mg/L共5个浓度,分别作用于大鼠肝癌细胞CBRH7919,72h后进行光镜下形态学观察,并采用四甲基偶氮唑盐(MTT)法测定癌细胞存活率。【结果】经初步分离的粗产品(浓度为0.2～25.0 mg/L)作用后的癌细胞存活率均低于相对应浓度的各对照组,72h半数抑制浓度(ρ_I_(50))为48.9 mg/L,并在25.0 mg/L以上浓度使癌细胞发生了明显的形态学改变。虫草素组72h的ρ_I_(50)为51.4mg/L。【结论】虫草素与钌形成配合物后对癌细胞生长抑制作用有所增强,并出现了特殊的形态改变。Objective To synthesize a novel ruthenium （Ⅱ） complex, 2-deoxyadenosine dipyidioruthenium （Ⅱ） with cordycepin as ligand, and to investigate its killing action on rat liver cancer cell strain CBRH7919. Methods With cordycepin as the main ligand and with 2＇, 2＇-bipyridine as the assisted ligand, the ruthenium complex was synthesized. After preliminary isolation and purification, a product of cordycepin, cis- [ Ru （bpy） 2 Cl2 ]·2H2O and the mixture of cordycepin and cis- [ Ru （bpy） 2 Cl2 ]·2H2O were obtained. CBRH7919 were incubated in vitro with the product at different concentrations of 0. 2, 1.0, 5.0, 25.0 and 125.0 mg/L, respectively. After incubation for 72 hours, morphological changes of CBRH were observed under inverted phase contrast microscope, and the cell survival rate was measured by methylthiazolyhetrazolium （MTT） assay. Results After the treatment of ruthenium （Ⅱ ） complex at the concentrations of 0.2 - 25.0 mg/L, cell survival rate was much lower than that in other groups at corresponding concentrations. IC50 of the ruthenium （Ⅱ） complex （72 h） was 48.9 mg/L. IC50 of cordycepin （72 h） was 51.4 mg/L. Additionally, remarkable morphological changes of the cells were found after the treatment of ruthenium （Ⅱ） complex at 25.0 - 125.0 mg/L. Conclusion The novel ruthenium （Ⅱ） complex with cordycepin has stronger inhibitory effect on the growth of liver cancer cells than cordycepin, and can induce specific morphological changes of the cancer cells.

关 键 词：肝肿瘤/中药疗法 肿瘤细胞/病理学 虫草素/化学合成 抗肿瘤试验 肿瘤细胞 培养的 

分 类 号：R284.3[医药卫生—中药学] R285.5[医药卫生—中医学]