益气健脾法对脾虚证模型大鼠血清胃泌素含量的影响  

Effect of Qi-activating and Spleen-strengthening Therapy on Serum Gastrin Content in Rats with Spleen-deficiency Syndrome

作　　者：曾益宏[1] 刘友章[1] 徐升[2] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广州中医药大学,2004级博士生广东广州510405

出　　处：《广州中医药大学学报》2008年第3期239-240,243,共3页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金(编号:30371706)

摘　　要：【目的】观察益气健脾法代表方剂对实验性脾虚证模型大鼠血清胃泌素(GAS)含量的影响。【方法】取SD大鼠60只随机分为6组,即正常对照组、脾虚模型组、四君子汤组、当归补血汤组、黄芪四君子汤组、当归补血汤合四君子汤组,采用破气苦降加饥饱失常法复制脾虚证大鼠模型,放射免疫法检测大鼠血清GAS的含量。【结果】脾虚模型组血清GAS含量显著性降低(P<0.05),各给药组均可显著性提高模型大鼠血清GAS含量(P<0.05),其作用大小依次为黄芪四君子汤组>当归补血汤合四君子汤组>四君子汤组>当归补血汤纽。【结论】益气健脾方药改善脾主运化功能的作用与其能升高血清胃泌素含量,促进胃肠道吸收有关。Objective To observe the effect of Qi-activating and spleen-strengthening therapy on serum gastrin （GAS） content in rats with spleen-deficiency system. Methods group, model group, Si Junzi Decoction （SJD） group, Danggui Sixty rats were randomized into 6 groups： normal Buxue Decoction （DBD） group, Huangqi Si Junzi Decoction （HSJD） group, and DBD ＋ SJD group. The rat model of spleen deficiency was induced by Qi-breaking bitter-taste herbs and alternation of starvation and gorge. Radioimmunological method was used to detect the serum GAS content. Results GAS content in the model group was decreased obviously （P 〈 0. 05 compared with the normal group）, and was increased in the medication groups （P 〈 0.05 compared with the model group）. The effect of HSJD, DBD ＋ SJD, SJD and DBD on the increase of GAS was in a decreasing sequence. Conclusion The therapeutic mechanism of Qi-activating and spleen-strengthening therapy for spleen-deficiency syndrome is related to the increase of serum GAS.

关 键 词：脾虚证/中药疗法 益气健脾法/治疗应用 胃泌素/血液 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]