白术种质AFLP指纹图谱分析体系的建立和应用  

Establishment and Application of AFLP Fingerprinting System in Atractylodes macrocephala Germplasm

作　　者：王志安[1] 徐行[2] 沈晓霞[1] 陈斌龙 寿惠霞[2] 

机构地区：[1]浙江省中药研究所,浙江杭州310023 [2]浙江大学生命科学学院,浙江杭州310058 [3]浙江省磐安县中药材研究所,浙江磐安322300

出　　处：《中药材》2008年第4期483-487,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:建立中药白术种质AFLP指纹图谱的分析体系。方法:10份野生及栽培白术样品,采用EcoRI/MseI双酶切和银染技术,以获得指纹图谱。结果:1)2倍CTAB法提取的白术基因组DNA质量最好;2)从40对AFLP引物中筛选到了16对能扩增出较强多态性的引物组合,共获得了3003条多态性条带,并根据多态位点可将10个白术种质聚类为四种类型。结论:建立的白术种质AFLP分析体系,可为种质保存和育种提供依据。Objective ：To establish the AFLP Fingerprinting system in the germplasm of Atractylodes macrocephala Koidz. Methods ： 10 wild or cultivated Atractylodes macrocephala were used for AFLP fingerprinting analysis by EcoRI and Msel restriction enzymes with silver staining. Results： Using 2X CTAB buffer extraction method can obtain the best genomic DNA samples. According to the AFLP polymorphism, sixteen out of forty primer pairs were selected to be suitable for AFLP analysis. Total 3003 polymorphic bands were obtained from the 16 sets of primer. Based on the AFLP results, the 10 samples of Atractylodes macrocephala germplasm were classified into four types. Conclusion ： The establishment of the AFLP fingerprinting system in Atractylodes macrocephala will be used in the identification of germplasms and breeding of the species.

关 键 词：白术 种质 AFLP 纹图谱 

分 类 号：R282.71[医药卫生—中药学]