积雪草苷对LPS诱导小鼠急性肺损伤炎症因子平衡的影响  

Effects of Asiaticoside on the Balance of Inflammatory Factors of Mouse's Acute Lung Injury Induced by LPS

作　　者：章卓[1] 秦大莲[1] 万敬员[2] 周岐新[2] 肖顺汉[1] 吴柯[2] 

机构地区：[1]泸州医学院药学院药理教研室,四川泸州646000 [2]重庆医科大学药学院药理教研室,重庆400016

出　　处：《中药材》2008年第4期547-549,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助课题(30500463)

摘　　要：目的:观察积雪草苷对脂多糖(LPS)诱导急性肺损伤支气管肺泡灌洗液(BALF)IL-6、TNF-α、IL-10的影响。方法:56只Balb/c小鼠随机分为空白、模型、假手术、溶媒、积雪草苷5,15,45mg/kg剂量组。LPS(2.5mg/kg)气管滴注造急性肺损伤模型,24h后处死小鼠,酶联免疫吸附法(ELISA)检测血浆IL-6、TNF-α、IL-10表达。结果:积雪草苷各剂量组呈剂量依赖性降低LPS所致急性肺损伤模型BALF中IL-6、TNF-α含量,增加IL-10表达。结论:积雪草苷对LPS诱导急性肺损伤保护作用可能与抑制炎症因子IL-6、TNF-α和促进IL-10,维持炎症因子间平衡有关。Objective： To observe the effect of asiaticoside （AS） on IL-6, TNF-α, IL-10 of bronchoaliveolar lavage fluid （BALF） of acute lung injury（ALI） induced by lipopolysaccharides （LPS）. Methods： 56 Balb/c mice were randomly divided into control , model, sham operation, vehicle, model ＋ asiaticoside 5,15,45 mg/kg groups. Model of ALI were established by intratracheal instillation of LPS 20μ1（2. 5 mg/kg）, mice were killed 24 hours later, the content of IL-6,TNF-α,IL-10 of BALF was detected by enzyme-linked immunosorbentassay （ELISA）. Results： AS could reduce the content of IL-6,TNF-α and increase IL-10 of BALF in dose-dependent manner. Conclusion： The protective effects against ALI induced by LPS on AS are related to reducing the content of IL-6,TNF-α, increasing secretion IL-10 and keeping the balance between inflammatory factors and anti-inflammatory factors.

关 键 词：积雪草苷 脂多糖 急性肺损伤 炎症因子 

分 类 号：R285.5[医药卫生—中药学]