半枝莲含药血清对小鼠肝癌细胞生长的抑制和诱导凋亡作用  

Anti-proliferative and Apoptosis-inducing Activity of Scutellaria barbate Containing Serum on Mouse's Hepatoma H22 Cells

作　　者：代志军[1] 刘小旭[1] 薛茜[2] 纪宗正[1] 王西京[1] 康华峰[1] 管海涛[1] 马小斌[1] 任宏涛[1] 

机构地区：[1]西安交通大学医学院第二附属医院肿瘤科,陕西西安710004 [2]第四军医大学基础部免疫学教研室,陕西西安710032

出　　处：《中药材》2008年第4期550-553,共4页Journal of Chinese Medicinal Materials

基　　金：陕西省科技攻关项目[2006K16-G5(1)];西安市科技创新支撑计划(YF07175)

摘　　要：目的:探讨中药半枝莲提取物(ESB)对小鼠肝癌细胞生长的抑制和诱导凋亡作用。方法:体外培养小鼠H22肝癌细胞,以ESB高、中、低剂量含药血清培养液作用于H22细胞,并设立空白血清及5-氟尿嘧啶组。应用MTT法检测药物对细胞增殖的抑制作用;Hoechst33258染色,荧光显微镜下观察细胞凋亡;流式细胞术检测肿瘤细胞周期及细胞凋亡情况。结果:ESB高、中剂量含药血清组有一定的抑制H22肝癌细胞增殖的作用,且呈明显的时效关系。荧光显微镜下可见部分细胞呈典型凋亡形态学改变。流式细胞仪检测结果显示:细胞周期各时相中,ESB高、中、剂量含药血清组S期细胞所占的比例明显减少,G1期细胞增多,有明显的诱导凋亡作用。其中空白对照、ESB低、中、高剂量组、5-氟尿嘧啶组H22细胞凋亡率分别为0.51%、1.07%、3.15%、7.83%、11.26%。结论:ESB含药血清不仅可直接抑制小鼠H22肝癌细胞生长,而且可有效诱导细胞凋亡。Objective ：To investigate the effects of Scutellaria barbate extract （ESB） on suppressing proliferation and inducing ap- optosis of mouse hepatoma H22 cells. Methods ： H22 cells cultured in vitro were divided into 5 groups ： blank control group, ESB in high, medium, low dose groups and 5-Fu group. H22 cells were cultured in media with serum containing different concentrations of ESB and blank serum. The proliferation of H22 cells was determined by microculture tetrazolium （MTT） assay. Fluorescence microscopy was utilized to observe the apoptosis of H22 cells by staining with Hoechst 33258. The cell cycle and apoptosis were analyzed by flow cytometry （FCM）. Results：The inhibition of serum containing ESB on the proliferation of H22 cells in vitro was observerd in a dose and time dependent manner. The typical morphological changes of apoptosis were observed after incubation with ESB-eontaining serum in high dose for 48 hours. Among the various phases of cell cycle, the percentage of cells in S phase decreased significantly, while the percentage of cells in G1 phase increased. Drug-containing serum showed positive effect on cell apoptosis. The apoptosis rate of blank eontrol group, ESB in low, medium, high dose groups and 5-Fu group were 0. 51% , 1.07% , 3.15% , 7. 83% , 11.26% , respectively. Conelusion：ESB containing serum ean inhibit proliferation and induce apoptosis of H22 cells in vitro.

关 键 词：半枝莲 肝癌 含药血清 凋亡 

分 类 号：R285.5[医药卫生—中药学]