茅莓根中对多巴胺神经元保护作用成分的分离鉴定研究  

Isolation and Identification of Suavissimoside R1 from Roots of Rubus parvifollus Used for Protecting Dopaminergic Neurons against MPP^+ Toxicity

作　　者：于占洋[1] 阮浩澜[2] 朱小南[2] 汪雪兰[2] 陈汝筑[2] 林永成[1] 

机构地区：[1]中山大学化学与化学工程学院,广东广州510275 [2]中山大学中山医学院药理教研室,广东广州510080

出　　处：《中药材》2008年第4期554-557,共4页Journal of Chinese Medicinal Materials

基　　金：中国人民解放军“九五”军队医药卫生科研基金(96M028);全军“九五”医学科研规划第二批科研基金(98M034);广东省中医药管理局科研基金(98483);广州市科技计划项目基金(2004Z3-E4131);广东省高等学校中药有效性与安全性重点实验室开放基金(Kf07004)

摘　　要：目的:从茅莓根中分离鉴定出具有保护多巴胺能神经元作用的甜叶苷R1(Suavissimoside R1)。方法:用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)处理的小鼠帕金森病模型检测从茅莓根中提取出的粗提物以及二次提取物(精提物)的保护作用,然后在此基础上对精提物进行分离纯化,最后分离出白色针状结晶性成分,根据化合物的理化常数测定和用红外光谱数据分析鉴定该成分结构为甜叶苷R1。以1-甲基-4-苯基吡啶离子(MPP+)处理的大鼠中脑多巴胺能神经元为模型,检测了甜叶苷R1对多巴胺能神经元的保护作用。结果:分离得到甜叶苷R1。甜叶苷R1浓度在100μmol/L时,对MPP+处理的多巴胺能神经元有明显的保护作用。结论:甜叶苷R1对多巴胺能神经元有保护作用。Objective： Suavissimoside R1 was isolated and identified as an active ingredient from Roots of Rubus parvifollus L. , which exhibited protective effect on dopaminergic neurons against MPP^＋ toxicity. Methods ： The protective effects of crude extracts were investigated after mice were treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine （MPTP）. According to the protective effects of crude extracts, suavissimoside R1, one kind of triterpenoid saponin, was separated. It was investigated that whether Suavissimoside R1 can protect DA neurons from toxicity induced by MPP^＋ in rat mesencephalic cultures. Results：Suavissimoside R1 was isolated from Roots of Rubus parvifollus L. Moreover, Suavissimoside R1, in dose of 100μmol/L, alleviated the death of DA neurons induced by MPP^＋ obviously. Conclusion ：These results suggest that suavissimoside R1 possesses potent neuroprotective activity and can be developed to be a potential anti-Parkinson＇s disease drug worthy for further study.

关 键 词：茅莓 甜叶苷R1 多巴胺能神经元 帕金森病 

分 类 号：R285.5[医药卫生—中药学] R284.2[医药卫生—中医学]