益气养阴、活血化瘀方对实验性病毒性扩张型心肌病小鼠心肌细胞凋亡的影响  

The Influence of Yiqiyangyin and Huoxuehuayu on Apoptosis of Myocardiocyte in Rats with Viral Dilated Cardiomyopathy

作　　者：周亚滨[1] 张丽丽[2] 高运吉[2] 张占军[2] 

机构地区：[1]黑龙江中医药大学附属第一医院 [2]黑龙江中医药大学

出　　处：《中西医结合心脑血管病杂志》2008年第5期540-541,共2页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：黑龙江省杰出青年基金项目(No.JC0208)

摘　　要：目的观察益气养阴、活血化瘀方对实验性病毒性扩张型心肌病小鼠心肌细胞凋亡的影响,探讨其防治病毒性扩张型心肌病的作用机制。方法采用多次腹腔注射柯萨奇B3m病毒的方式,建立重复感染的早期病毒性扩张型心肌病动物模型。将BALB/c小鼠210只随机分为4组,空白组30只,模型组、中药高剂量组、中药低剂量组各60只。中药高剂量组、中药低剂量组用益气养阴、活血化瘀方治疗4周后,采用原位末端标记法(TUNEL),观察益气养阴、活血化瘀方对实验性病毒性扩张型心肌病小鼠心肌细胞凋亡的影响。结果中药高、低剂量组与模型组相比,可改善小鼠心肌组织病理形态学及超微结构的变化,抑制心肌细胞凋亡。结论益气养阴、活血化瘀方能够减轻病毒性扩张型心肌病小鼠的病理损伤,抑制心肌细胞肥大,抑制心肌细胞凋亡,从而对病毒性扩张型心肌病起到一定的防治作用。Objective To study the effect of yiqiyangyin and huoxuehuayu on apoptosis of myocardiocyte in rats with viral dilated cardiomyopathy. Methods The animal models of the early viral dilated cardiomyopathy were replicated with injection of the CVB3 m virus in the peritoneal cavity of the BALB/c rats. Two hundred and ten BALB/c rats were divided into 4 groups at random： Blank group （ n = 30）, high dose traditional Chinese medicine group （ n = 60） and low dose traditional Chinese medicine group （n = 60） with Yiqiyangyinfang and Huoxuehuayu for 4 weeks. TUNEL method was used to observe the effect of yiqiyangyinfang and huoxuehuayu on the rat model with viral dilated cardiomyopathy. Results Comparison with model group, the prescription of yiqiyangyin and huoxuehuayu in high dose traditional Chinese medicine group and low dose traditional Chinese medicine group could improve the pathomorphism and the ultrastructure of myocardiocyte in rats with viral dilated cardiomyopathy and inhabit apoptosis of myoeardium. Conclusion The prescription of yiqiyangyin and huoxuehuayu could alleviate the pathologic injury and inhabit apoptosis of myocardium.

关 键 词：益气养阴、活血化瘀方 病毒性扩张型心肌病 心肌细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]