离子色谱法测定沙坦类药物中的叠氮酸根  

Determination of azide in Sartan by ion chromatography

作　　者：姚超英[1] 

机构地区：[1]杭州职业技术学院化工系

出　　处：《浙江大学学报（理学版）》2008年第3期305-307,310,共4页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金资助项目(20375035);浙江省自然科学基金资助项目(Z404105)

摘　　要：选用IonPacAS18离子色谱柱分离，利用在线淋洗液发生器自动产生氢氧化钾梯度淋洗液，以抑制电导降低淋洗液的背景电导进行沙坦类药物中叠氮化钠含量的测定．该方法可以将药物中的各类杂质有效分离，简化了样品的预处理，提高了测定的灵敏度．研究表明：叠氮根的检出限（S／N=3）为0．23Fg·L^-1，检测的线性范围在0．001～20mg·L^-1，其相关系数为r^2〉0．9992．采用该方法测定了厄贝沙坦，氯沙坦试样及中间体保护基溴化物中的叠氮化钠含量，结果令人满意．The assay for azide was recently improved by using ion chromatography on an IonPac AS18 ion exchange column and an eluent generator that automatically generates potassium hydroxide as mobile phase and suppresses conductivity detection. Azide can successfully separate from other common species in Sartan. There was also no interference from other common anions （fluoride, bromide, chloride, nitrite, phosphate and sulfate） in this investigation. The sensitivity was significantly improved by RFIC as compared with that of a system using sodium carbonate elutions attributed to the extremely low background conductivity water. Therefore, unlike the sodium carbonate system in which samples were steam distilled to improve the determination precision, the method we described was much more simple and effective with easier sample pretreatment. The calibration graphs of analytes monitored on IonPac AS18 column was linear in the range of 0. 001-20 mg·L^-1 （r^2〉0. 999 2） for azide. The mean of detection limits of azide was 0.23 μg· L^-1（S/N= 3）. Good linear relationship, sensitivity, and reproducibility were obtained.

关 键 词：叠氮根 离子色谱 厄贝沙坦 氯沙坦 

分 类 号：O657.7[理学—分析化学]