体细胞核重编程中表观遗传学的研究进展  

The Progress of Epigenetics in Somatic Nuclear Reprogramming

作　　者：关娜[1] 徐燕宁[1] 张庆华[1] 雷蕾[1] 

机构地区：[1]哈尔滨医科大学组织学与胚胎学教研室,哈尔滨150081

出　　处：《生物化学与生物物理进展》2008年第5期496-501,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(30671025);哈尔滨医科大学基础医学学院科研基金资助项目~~

摘　　要：促使体细胞核重编程的方法很多,除了传统的体细胞核移植方法外,科学家们努力寻求从法律、道德、伦理等方面更易被人们接受的新方法.近年来多能干细胞与体细胞融合、多能细胞的抽提物与体细胞共孵育以及将编码多潜能因子的基因导入体细胞中等方法都能使体细胞核发生重新编程,将已分化的体细胞转变为一种全能的胚胎状态.主要论述了生殖细胞及早期胚胎、体细胞核移植和其他形式的体细胞核重编程的表观遗传学的改变,对表观遗传学的深入研究将有助于我们进一步了解体细胞核重编程的机制,从而不断完善各种技术促进供体核的重新编程,使其更好地应用于基础研究和生产实践.Many Novel approaches of epigenetic reprogramming of somatic cells have been reported. However, ethical issues caused by somatic nuclear transfer have triggered the development of alternative strategies for reprogramming somatic cells. Recently, many new advances have been acquired for reprogramming somatic cells, which could reverse differentiated somatic cells to a totipotent embryonic state, such as fusion of potential stem cells with somatic cells, incubation of cells in potential cell-free extraction and introduction of defined pluripotency factors into somatic cells. The epigenetic modification in these reprogramming processes, including germ cells and early embryoes, somatic nuclear transfer and other approaches for reprogramming of somatic cells were reviewed. Studies of epigenetics will be benefit for understanding the precise mechanism and improving the efficiency of somatic nuclear reprogramming, which will be eventually applied in the basic study and practice.

关 键 词：重编程 表观遗传学 甲基化 

分 类 号：Q291[生物学—细胞生物学]